dimethyl-Histone H3 (Lys4) Antibody, clone AW30, rabbit monoclonal

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

dimethyl-Histone H3 (Lys4) Antibody, clone AW30, rabbit monoclonal

catalog :

04-790

quantity :

100 µL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

AW30

reactivity :

human

application :

western blot, chromatin immunoprecipitation

citations: 4

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; human	Bazot Q, Deschamps T, Tafforeau L, Siouda M, Leblanc P, Harth Hertle M, et al. Epstein-Barr virus nuclear antigen 3A protein regulates CDKN2B transcription via interaction with MIZ-1. Nucleic Acids Res. 2014;42:9700-16 pubmed publisher
	Higgs M, Sato K, Reynolds J, Begum S, Bayley R, Goula A, et al. Histone Methylation by SETD1A Protects Nascent DNA through the Nucleosome Chaperone Activity of FANCD2. Mol Cell. 2018;71:25-41.e6 pubmed publisher
	Chen Q, Chen X, Wang Q, Zhang F, Lou Z, Zhang Q, et al. Structural basis of a histone H3 lysine 4 demethylase required for stem elongation in rice. PLoS Genet. 2013;9:e1003239 pubmed publisher
	Li W, Han Y, Tao F, Chong K. Knockdown of SAMS genes encoding S-adenosyl-l-methionine synthetases causes methylation alterations of DNAs and histones and leads to late flowering in rice. J Plant Physiol. 2011;168:1837-43 pubmed publisher

product information

Catalog Number :

04-790

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-dimethyl-Histone H3 (Lys4) Antibody, clone AW30, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q16695

Host Name :

Rabbit

Antigen :

dimethyl-Histone H3 (Lys4)

Conjugate :

Culture Supernatant

Isotype :

IgG

Product Description :

Anti-dimethyl-Histone H3 (Lys4) Antibody, clone AW30, rabbit monoclonal

Cross Reactivity :

Human;Chicken

Background :

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Di- and trimethylation of histone H3 at Lys4 correlates with transcriptional activity of many genes. Dimethylation of Histone H3 at Lys4 occurs at both active and inactive euchromatic regions but not in silent heterochromatic sites, whereas trimethylation at Lys4 is present exclusively at active genes.

ALT Names :

H3K4me2;Histone H3 (di methyl K4)

Immunogen :

KLH-conjugated, synthetic peptide containing the sequence …Tme2KQT… in which me2K corresponds to dimethyl-lysine at residue 4 of human histone H3.

Specificity :

Histone H3 dimethylated on lysine 4

Package Size :

100 µL

Uses :

Western Blotting;ChIP-seq;Chromatin Immunoprecipitation (ChIP);Dot Blot;Peptide Inhibition Assay

Storage :

Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.