phospho-Histone H3 (Thr11) Antibody, clone MC83, rabbit monoclonal

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-Histone H3 (Thr11) Antibody, clone MC83, rabbit monoclonal

catalog :

04-789

quantity :

200 µL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

MC83

reactivity :

human

application :

western blot

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:2000	Egelhofer T, Minoda A, Klugman S, Lee K, Kolasinska Zwierz P, Alekseyenko A, et al. An assessment of histone-modification antibody quality. Nat Struct Mol Biol. 2011;18:91-3 pubmed publisher

product information

Catalog Number :

04-789

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-phospho-Histone H3 (Thr11) Antibody, clone MC83, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q16695

Host Name :

Rabbit

Antigen :

phospho-Histone H3 (Thr11)

Clone :

MC83

Isotype :

IgG

Product Description :

Anti-phospho-Histone H3 (Thr11) Antibody, clone MC83, rabbit monoclonal

Cross Reactivity :

Human

Background :

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications, which include acetylation, phosphorylation, methylation, and ubiquitin-ation, occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Phosphorylation at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. In mammals, phosphorylation of Thr11 modification is restricted to the centromeric region while in plant cells, phosphorylation of Thr11 was distributed along the entire length of condensed chromosomes. Mitosis-specific phosphorylation of histone H3 at Thr11 is mediated by Dlk/ZIP kinase.

ALT Names :

H3T11P;Histone H3 (phospho T11)

Immunogen :

Peptide containing . KS[pT]G. , in which pT corresponds to phospho-threonine at position 11 of human histone H3

Specificity :

Recognizes histone H3 phosphorylated at Thr11, Mr 17kDa.

Package Size :

200 µL

Uses :

Western Blotting;Peptide Inhibition Assay;Multiplexing

Storage :

Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.