Ha-Ras Antibody, clone MC57, rabbit monoclonal | EMD Millipore 04-775 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Ha-Ras Antibody, clone MC57, rabbit monoclonal

catalog :

04-775

quantity :

100 µL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

MC57

reactivity :

human, mouse

application :

western blot

product information

Catalog Number :

04-775

Subcategory :

Cell Signaling

Product Name :

Anti-Ha-Ras Antibody, clone MC57, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P01112

Host Name :

Rabbit

Antigen :

Ha-Ras

Clone :

MC57

Isotype :

IgG

Product Description :

Anti-Ha-Ras Antibody, clone MC57, rabbit monoclonal

Cross Reactivity :

Human;Mouse;Rat

Background :

Ras proteins are small GTP-binding proteins which, unlike the heterotrimeric G-proteins, contain all GTPase and effector functions within a single polypeptide. At least three isoforms of Ras exist, Ki-Ras, Ha-Ras and N-Ras, with distinct expression patterns but similar signaling activity. Ras is palmitoylated and farnesylated at the carboxy terminus, anchoring it in the membrane. In resting cells, Ras is loaded with GDP, and is activated subsequent to growth factor stimulation of receptors, which recruit Ras Guanine nucleotide Exchange Factors to the plane of the membrane. Proximity of exchange factors to the Ras proteins causes release of GDP, and its replacement by GTP. In its GTP-bound form, Ras binds several proteins, including Raf, RalGDS and PI3 Kinase. Inactivation of Ras occurs by GTP hydrolysis, which is greatly accelerated by RasGAP or NF-1, two known Ras GTPase Activating Proteins. It is possible to assay for Ras activation by incubation of lysates with the Ras-binding domain of Raf-1, which selectively binds to Ras:GTP.

ALT Names :

GTP- and GDP-binding peptide B;GTPase HRas;Ha-Ras1 proto-oncoprotein;Ras family small GTP binding protein H-Ras;Transforming protein p21;c-has/bas p21 protein;c-ras-Ki-2 activated oncogene;p19 H-RasIDX protein;transformation gene: oncogene HAMSV;v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Immunogen :

GST fusion protein corresponding to full length human Ha-Ras. Clone MC57.

Specificity :

Recognizes Ha-Ras, Mr ~21 kDa. Does not cross react with Cdc42, Rho or Rac1.

Package Size :

100 µL

Uses :

Western Blotting

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.