phospho-MYPT1 (Thr850) Antibody,, rabbit monoclonal | EMD Millipore 04-773 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-MYPT1 (Thr850) Antibody,, rabbit monoclonal

catalog :

04-773

quantity :

100 μl

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SA19

reactivity :

human, mouse, chicken

application :

western blot

citations: 1

Reference
Kogata N, Tribe R, Fassler R, Way M, Adams R. Integrin-linked kinase controls vascular wall formation by negatively regulating Rho/ROCK-mediated vascular smooth muscle cell contraction. Genes Dev. 2009;23:2278-83 pubmed publisher

product information

Catalog Number :

04-773

Subcategory :

Cell Signaling

Product Name :

Anti-phospho-MYPT1 (Thr850) Antibody,, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

O14974

Host Name :

Rabbit

Antigen :

phospho-MYPT1 (Thr850)

Clone :

SA19

Isotype :

IgG

Product Description :

Anti-phospho-MYPT1 (Thr850) Antibody,, rabbit monoclonal

Cross Reactivity :

Chicken;Human;Mouse;Rat

Background :

Phosphorylation of myosin is crucial to smooth muscle contraction. The degree of myosin phosphorylation is dependent in part by the activity of myosin phosphatase. Recent findings suggest that this enzyme can be regulated, which has lead to interest in characterizing this phosphatase. Myosin phosphatase seems to be comprised of three subunits (catalytic subunit of type 1 phosphatase-δ (PP1) and two non-catalytic subunits, large and small (M20)). Studies suggest that the large subunit is a targeting subunit and is termed myosin phosphatase target subunit (MYPT). MYPT has been shown to be a substrate for the Rho-associated kinase (ROK). ROK mediates an agonist-induced increase in myosin phosphorylation and force by inhibiting myosin light chain phosphatase activity through phosphorylation of MYPT.

ALT Names :

Myosin phosphatase target subunit 1;Myosin phosphatase-targeting subunit 1;Protein phosphatase myosin-binding subunit;myosin phosphatase, target subunit 1;protein phosphatase 1, regulatory (inhibitor) subunit 12A

Immunogen :

KLH-conjugated, synthetic peptide containing the sequence …RS[pT]GV…, in which [pT] corresponds to phosphorylated threonine at amino acid residue 850 of chicken myosin phosphatase target subunit 1 (MYPT1). Corresponds to Thr853 in human MYPT1.

Specificity :

Recognizes MYPT1 phosphorylated on Thr850, doublet at Mr ~130 kDa. A non-specific protein was also detected, Mr ~55-60 kDa.

Package Size :

100 μl

Uses :

Western Blotting

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.