Akt2/PKBβ Antibody, clone AW114, rabbit monoclonal | EMD Millipore 04-771 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Akt2/PKBβ Antibody, clone AW114, rabbit monoclonal

catalog :

04-771

quantity :

100 μl

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

AW114

reactivity :

human, mouse

application :

western blot, immunoprecipitation

product information

Catalog Number :

04-771

Subcategory :

Cell Signaling

Product Name :

Anti-Akt2/PKBβ Antibody, clone AW114, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P31751

Host Name :

Rabbit

Antigen :

Akt2/PKBβ

Clone :

AW114

Isotype :

IgG

Product Description :

Anti-Akt2/PKBβ Antibody, clone AW114, rabbit monoclonal

Cross Reactivity :

Human;Mouse;Rat

Background :

Akt is the major known effector of the PI3 Kinase pathway. Generation of PIP3 results in the activation of PDK1, which phosphorylates Akt on Thr308, and another kinase complex, thought to be the mTORC2 complex (or possibly an intermediate kinase linking the two) that phosphorylates Akt on Ser473. These phosphorylations additively activate Akt Ser/Thr kinase activity, and the use of phosphorylation state-specific antibodies directed against either of these sites can imply Akt activation. Activation of Akt can be measured directly by immunoprecipitation followed by phosphorylation of a known substrate with radiolabeled ATP. Akt has been shown to phosphorylate over 70 substrates including TSC1&2 in the mTOR pathway, Bad on Ser136, GSK3 (which is inactivated by this phosphorylation), and the FoxO family of transcription factors (PRAS40, AS160).

ALT Names :

AKT2 kinase ;Murine thymoma viral (v-akt) homolog-2;PKB beta;Protein kinase Akt-2;Protein kinase B, beta ;rac protein kinase beta;v-akt murine thymoma viral oncogene homolog 2

Immunogen :

KLH-conjugated, synthetic peptide (CRYDSLGSLELDQRTH) corresponding to amino acids 455-469 of human Akt2/PKBβ, with an N-terminal cysteine added for conjugation purposes.

Specificity :

Recognizes Akt2/PKBβ, Mr ~60 kDa.

Package Size :

100 μl

Uses :

Western Blotting;Immunoprecipitation

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.