phospho-MEK1 (Ser218/222)/ MEK2 (Ser222/226) | EMD Millipore 04-747 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-MEK1 (Ser218/222)/ MEK2 (Ser222/226)

catalog :

04-747

quantity :

100 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot

product information

Catalog Number :

04-747

Category :

Antibodies

Subcategory :

Cell Signaling

Product Name :

Anti-phospho-MEK1 (Ser218/222)/ MEK2 (Ser222/226)

Product Type :

Antibodies

Clonality :

Monoclonal

Gene ID :

NP_002746.1, NP_109587.1

Host Name :

Isotype :

IgG

Product Description :

Anti-phospho-MEK1 (Ser218/222)/ MEK2 (Ser222/226)

Cross Reactivity :

Human, Mouse, Rat

Background :

MAP Kinase/Erk Kinase (MEK), alternatively known as MKK, is a true dual-specificity kinase, in that it phosphorylates the MAP kinases on both the Thr and Tyr of the activation motif TEY. In vitro, the tyrosine phosphorylation is favored, whereas In vivo both phosphorylation events appear to occur simultaneously. This suggests that an additional factor is present in cells to facilitate the reaction. MEK1 and MEK2 are activated by phosphorylation of two serine residues (Ser218/222 in MEK1 and Ser222/226 in MEK2), which are substrates for the Raf family of kinases. Mutation of the phosphorylation sites from Ser to Asp creates a protein with constitutive kinase activity, which when expressed in cells is able to cause transformation.

ALT Names :

ERK activator kinase 1, MAP kinase kinase 1, MAPK/ERK kinase 1, MAPKK 1, mitogen-activated protein kinase kinase 1 , protein kinase, mitogen-activated, kinase 1 (MAP kinasekinase 1)

Immunogen :

Peptide containing DpSMANpSF in which pS corresponds to phosphorylated amino acids 218 and 222 of human MEK1.

Specificity :

Phosphorylated MEK1/MEK2 at Ser218/222 and Ser222/226 respectively.

Package Size :

100 ul

Uses :

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.