phospho-Histone H3 (Thr3) Antibody, clone JY325, rabbit monoclonal

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-Histone H3 (Thr3) Antibody, clone JY325, rabbit monoclonal

catalog :

04-746

quantity :

100 µL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JY325

reactivity :

human, fruit fly

application :

western blot, immunohistochemistry, immunoprecipitation

citations: 4

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; fruit fly ; 1:100; loading ...; fig s2h	Kim J, Lu C, Srinivasan S, Awe S, Brehm A, Fuller M. Blocking promiscuous activation at cryptic promoters directs cell type-specific gene expression. Science. 2017;356:717-721 pubmed publisher
	Huertas D, Soler M, Moretó J, Villanueva A, Martinez A, Vidal A, et al. Antitumor activity of a small-molecule inhibitor of the histone kinase Haspin. Oncogene. 2012;31:1408-18 pubmed publisher
	Xu C, Cole P, Meyers D, Kormish J, Dent S, Zaret K. Chromatin "prepattern" and histone modifiers in a fate choice for liver and pancreas. Science. 2011;332:963-6 pubmed publisher
	Patnaik D, Jun Xian -, Glicksman M, Cuny G, Stein R, Higgins J. Identification of small molecule inhibitors of the mitotic kinase haspin by high-throughput screening using a homogeneous time-resolved fluorescence resonance energy transfer assay. J Biomol Screen. 2008;13:1025-34 pubmed publisher

product information

Catalog Number :

04-746

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-phospho-Histone H3 (Thr3) Antibody, clone JY325, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q16695

Host Name :

Rabbit

Antigen :

phospho-Histone H3 (Thr3)

Clone :

JY325

Conjugate :

Culture Supernatant

Isotype :

IgG

Product Description :

Anti-phospho-Histone H3 (Thr3) Antibody, clone JY325, rabbit monoclonal

Cross Reactivity :

Human

Background :

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

ALT Names :

H3T3P;Histone H3 (phospho T3)

Immunogen :

Peptide containing the sequence [RT trim KQ] in which lysine 4 is trimethylated on human histone H3

Specificity :

Histone H3 phosphorylated at Threonine 3; cross-reactivity with phospho-histone H3 Thr22 detected in some applications

Package Size :

100 µL

Uses :

Western Blotting;Dot Blot;Multiplexing;Peptide Inhibition Assay

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.