This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
trimethyl-Histone H3 (Lys4) Antibody, clone MC315, rabbit monoclonal
catalog :
04-745
quantity :
100 µL
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
MC315
reactivity :
African green monkey, Saccharomyces cerevisiae S288C, human, mouse, chicken
application :
western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, ChIP-Seq
citations: 80
Published Application/Species/Sample/DilutionReference
  • ChIP-Seq; human; fig 1d
Casanova M, Moscatelli M, Chauvière L, Huret C, Samson J, Liyakat Ali T, et al. A primate-specific retroviral enhancer wires the XACT lncRNA into the core pluripotency network in humans. Nat Commun. 2019;10:5652 pubmed publisher
  • chromatin immunoprecipitation; mouse; loading ...; fig 3a
Klein R, Tung P, Somanath P, Fehling H, Knoepfler P. Genomic functions of developmental pluripotency associated factor 4 (Dppa4) in pluripotent stem cells and cancer. Stem Cell Res. 2018;31:83-94 pubmed publisher
  • ChIP-Seq; human; loading ...
van Groningen T, Koster J, Valentijn L, Zwijnenburg D, Akogul N, Hasselt N, et al. Neuroblastoma is composed of two super-enhancer-associated differentiation states. Nat Genet. 2017;49:1261-1266 pubmed publisher
  • chromatin immunoprecipitation; mouse; loading ...; fig 4b
Mathew R, Tatarakis A, Rudenko A, Johnson Venkatesh E, Yang Y, Murphy E, et al. A microRNA negative feedback loop downregulates vesicle transport and inhibits fear memory. elife. 2016;5: pubmed publisher
  • western blot; mouse; loading ...; fig 3a
  • western blot; human; loading ...; fig 3a
Zhong J, Li X, Cai W, Wang Y, Dong S, Yang J, et al. TET1 modulates H4K16 acetylation by controlling auto-acetylation of hMOF to affect gene regulation and DNA repair function. Nucleic Acids Res. 2017;45:672-684 pubmed publisher
  • immunoprecipitation; human; fig 2
  • western blot; mouse; fig 4
Chung H, Xu C, Fuchs A, Mund A, Lange M, Staege H, et al. PHF13 is a molecular reader and transcriptional co-regulator of H3K4me2/3. elife. 2016;5: pubmed publisher
  • ChIP-Seq; mouse; fig 1
Goode D, Obier N, Vijayabaskar M, Lie A Ling M, Lilly A, Hannah R, et al. Dynamic Gene Regulatory Networks Drive Hematopoietic Specification and Differentiation. Dev Cell. 2016;36:572-87 pubmed publisher
  • chromatin immunoprecipitation; human; fig 6
Theiss J, Gunther T, Alawi M, Neumann F, Tessmer U, Fischer N, et al. A Comprehensive Analysis of Replicating Merkel Cell Polyomavirus Genomes Delineates the Viral Transcription Program and Suggests a Role for mcv-miR-M1 in Episomal Persistence. PLoS Pathog. 2015;11:e1004974 pubmed publisher
  • chromatin immunoprecipitation; human; fig 5b
Lee Y, Li Y, Su C, Chiao C, Lin I, Hsu M. MAF1 represses CDKN1A through a Pol III-dependent mechanism. elife. 2015;4:e06283 pubmed publisher
  • chromatin immunoprecipitation; human; fig 2
Meidhof S, Brabletz S, Lehmann W, Preca B, Mock K, Ruh M, et al. ZEB1-associated drug resistance in cancer cells is reversed by the class I HDAC inhibitor mocetinostat. EMBO Mol Med. 2015;7:831-47 pubmed publisher
  • ChIP-Seq; human; fig s7
Xu J, Shao Z, Li D, Xie H, Kim W, Huang J, et al. Developmental control of polycomb subunit composition by GATA factors mediates a switch to non-canonical functions. Mol Cell. 2015;57:304-316 pubmed publisher
  • chromatin immunoprecipitation; mouse; fig 5
Tusi B, Deng C, Salz T, Zeumer L, Li Y, So C, et al. Setd1a regulates progenitor B-cell-to-precursor B-cell development through histone H3 lysine 4 trimethylation and Ig heavy-chain rearrangement. FASEB J. 2015;29:1505-15 pubmed publisher
  • ChIP-Seq; mouse
Qian J, Wang Q, Dose M, Pruett N, Kieffer Kwon K, Resch W, et al. B cell super-enhancers and regulatory clusters recruit AID tumorigenic activity. Cell. 2014;159:1524-37 pubmed publisher
  • immunocytochemistry; human
Luense S, Denner P, Fernández Montalván A, Hartung I, Husemann M, Stresemann C, et al. Quantification of histone H3 Lys27 trimethylation (H3K27me3) by high-throughput microscopy enables cellular large-scale screening for small-molecule EZH2 inhibitors. J Biomol Screen. 2015;20:190-201 pubmed publisher
  • chromatin immunoprecipitation; human; fig 5
  • western blot; human; fig 1
Salz T, Deng C, Pampo C, Siemann D, Qiu Y, Brown K, et al. Histone Methyltransferase hSETD1A Is a Novel Regulator of Metastasis in Breast Cancer. Mol Cancer Res. 2015;13:461-9 pubmed publisher
  • chromatin immunoprecipitation; human
Moquet Torcy G, Tolza C, Piechaczyk M, Jariel Encontre I. Transcriptional complexity and roles of Fra-1/AP-1 at the uPA/Plau locus in aggressive breast cancer. Nucleic Acids Res. 2014;42:11011-24 pubmed publisher
  • chromatin immunoprecipitation; human
Liefke R, Borggrefe T. Probing the epigenetic status at Notch target genes. Methods Mol Biol. 2014;1187:255-76 pubmed publisher
  • chromatin immunoprecipitation; mouse
Veazey K, Carnahan M, Muller D, Miranda R, Golding M. Alcohol-induced epigenetic alterations to developmentally crucial genes regulating neural stemness and differentiation. Alcohol Clin Exp Res. 2013;37:1111-22 pubmed publisher
  • chromatin immunoprecipitation; chicken
Kohler K, McDonald J, Duke J, Arakawa H, Tan S, Kleinstein S, et al. Identification of core DNA elements that target somatic hypermutation. J Immunol. 2012;189:5314-26 pubmed publisher
  • chromatin immunoprecipitation; Saccharomyces cerevisiae S288C
Weiner A, Chen H, Liu C, Rahat A, Klien A, Soares L, et al. Systematic dissection of roles for chromatin regulators in a yeast stress response. PLoS Biol. 2012;10:e1001369 pubmed publisher
  • chromatin immunoprecipitation; African green monkey
Milavetz B, Kallestad L, Gefroh A, Adams N, Woods E, Balakrishnan L. Virion-mediated transfer of SV40 epigenetic information. Epigenetics. 2012;7:528-34 pubmed publisher
  • chromatin immunoprecipitation; mouse
Weishaupt H, Attema J. A Method to Study the Epigenetic Chromatin States of Rare Hematopoietic Stem and Progenitor Cells; MiniChIP-Chip. Biol Proced Online. 2010;12:1-17 pubmed publisher
  • chromatin immunoprecipitation; human
  • western blot; human; 1:2000
Egelhofer T, Minoda A, Klugman S, Lee K, Kolasinska Zwierz P, Alekseyenko A, et al. An assessment of histone-modification antibody quality. Nat Struct Mol Biol. 2011;18:91-3 pubmed publisher
  • chromatin immunoprecipitation; human
Kumari D, Biacsi R, Usdin K. Repeat expansion affects both transcription initiation and elongation in friedreich ataxia cells. J Biol Chem. 2011;286:4209-15 pubmed publisher
Liu X, Chen Y, Zhang Y, Liu Y, Liu N, Botten G, et al. Multiplexed capture of spatial configuration and temporal dynamics of locus-specific 3D chromatin by biotinylated dCas9. Genome Biol. 2020;21:59 pubmed publisher
Li Y, Liao Z, Luo H, Benyoucef A, Kang Y, Lai Q, et al. Alteration of CTCF-associated chromatin neighborhood inhibits TAL1-driven oncogenic transcription program and leukemogenesis. Nucleic Acids Res. 2020;48:3119-3133 pubmed publisher
Hanasaki M, Masumoto H. CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner. Sci Rep. 2019;9:15300 pubmed publisher
Mukai J, Cannavó E, Crabtree G, Sun Z, Diamantopoulou A, Thakur P, et al. Recapitulation and Reversal of Schizophrenia-Related Phenotypes in Setd1a-Deficient Mice. Neuron. 2019;104:471-487.e12 pubmed publisher
Ishiuchi T, Ohishi H, Sato T, Kamimura S, Yorino M, Abe S, et al. Zfp281 Shapes the Transcriptome of Trophoblast Stem Cells and Is Essential for Placental Development. Cell Rep. 2019;27:1742-1754.e6 pubmed publisher
Wang H, Long Q, Tang S, Xiao Q, Gao C, Zhao Q, et al. Histone demethylase KDM3A is required for enhancer activation of hippo target genes in colorectal cancer. Nucleic Acids Res. 2019;47:2349-2364 pubmed publisher
Edginton White B, Cauchy P, Assi S, Hartmann S, Riggs A, Mathas S, et al. Global long terminal repeat activation participates in establishing the unique gene expression programme of classical Hodgkin lymphoma. Leukemia. 2019;33:1463-1474 pubmed publisher
Kumar M, Kasti K, Balakrishnan L, Milavetz B. Directed Nucleosome Sliding during the Formation of the Simian Virus 40 Particle Exposes DNA Sequences Required for Early Transcription. J Virol. 2019;93: pubmed publisher
Yang C, Shen W, Chen H, Chu L, Xu Y, Zhou X, et al. Characterization and subcellular localization of histone deacetylases and their roles in response to abiotic stresses in soybean. BMC Plant Biol. 2018;18:226 pubmed publisher
Link V, Duttke S, Chun H, Holtman I, Westin E, Hoeksema M, et al. Analysis of Genetically Diverse Macrophages Reveals Local and Domain-wide Mechanisms that Control Transcription Factor Binding and Function. Cell. 2018;173:1796-1809.e17 pubmed publisher
Ali M, Ajore R, Wihlborg A, Niroula A, Swaminathan B, Johnsson E, et al. The multiple myeloma risk allele at 5q15 lowers ELL2 expression and increases ribosomal gene expression. Nat Commun. 2018;9:1649 pubmed publisher
Simon L, Rabanal F, Dubos T, Oliver C, Lauber D, Poulet A, et al. Genetic and epigenetic variation in 5S ribosomal RNA genes reveals genome dynamics in Arabidopsis thaliana. Nucleic Acids Res. 2018;46:3019-3033 pubmed publisher
Li Y, Zheng H, Wang Q, Zhou C, Wei L, Liu X, et al. Genome-wide analyses reveal a role of Polycomb in promoting hypomethylation of DNA methylation valleys. Genome Biol. 2018;19:18 pubmed publisher
Xu M, Leichty A, Hu T, Poethig R. H2A.Z promotes the transcription of MIR156A and MIR156C in Arabidopsis by facilitating the deposition of H3K4me3. Development. 2018;145: pubmed publisher
Vo L, Kinney M, Liu X, Zhang Y, Barragan J, Sousa P, et al. Regulation of embryonic haematopoietic multipotency by EZH1. Nature. 2018;553:506-510 pubmed publisher
Kaushik S, Liu F, Veazey K, Gao G, Das P, Neves L, et al. Genetic deletion or small-molecule inhibition of the arginine methyltransferase PRMT5 exhibit anti-tumoral activity in mouse models of MLL-rearranged AML. Leukemia. 2018;32:499-509 pubmed publisher
Bonev B, Mendelson Cohen N, Szabo Q, Fritsch L, Papadopoulos G, Lubling Y, et al. Multiscale 3D Genome Rewiring during Mouse Neural Development. Cell. 2017;171:557-572.e24 pubmed publisher
Huggins I, Bos T, Gaylord O, Jessen C, Lonquich B, Puranen A, et al. The WNT target SP5 negatively regulates WNT transcriptional programs in human pluripotent stem cells. Nat Commun. 2017;8:1034 pubmed publisher
Li H, Liefke R, Jiang J, Kurland J, Tian W, Deng P, et al. Polycomb-like proteins link the PRC2 complex to CpG islands. Nature. 2017;549:287-291 pubmed publisher
Joung J, Engreitz J, Konermann S, Abudayyeh O, Verdine V, Aguet F, et al. Genome-scale activation screen identifies a lncRNA locus regulating a gene neighbourhood. Nature. 2017;548:343-346 pubmed publisher
Jih G, Iglesias N, Currie M, Bhanu N, Paulo J, Gygi S, et al. Unique roles for histone H3K9me states in RNAi and heritable silencing of transcription. Nature. 2017;547:463-467 pubmed publisher
Kim J, Hu Z, Cai L, Li K, Choi E, Faubert B, et al. CPS1 maintains pyrimidine pools and DNA synthesis in KRAS/LKB1-mutant lung cancer cells. Nature. 2017;546:168-172 pubmed publisher
Kim J, Jin H, Zhao J, Yang Y, Li Y, Yang X, et al. FOXA1 inhibits prostate cancer neuroendocrine differentiation. Oncogene. 2017;36:4072-4080 pubmed publisher
Fogarty E, Brewer M, Rodriguez Molina J, Law W, Ma K, Steinberg N, et al. SOX10 regulates an alternative promoter at the Charcot-Marie-Tooth disease locus MTMR2. Hum Mol Genet. 2016;25:3925-3936 pubmed publisher
Hsu C, Wu P, Chen T, Yu C, Tsai W, Wu K, et al. Histone acetylation accompanied with promoter sequences displaying differential expression profiles of B-class MADS-box genes for phalaenopsis floral morphogenesis. PLoS ONE. 2014;9:e106033 pubmed publisher
Xue M, Long J, Jiang Q, Wang M, Chen S, Pang Q, et al. Distinct patterns of the histone marks associated with recruitment of the methionine chain-elongation pathway from leucine biosynthesis. J Exp Bot. 2015;66:805-12 pubmed publisher
Ellison T, Kedes D. Variable episomal silencing of a recombinant herpesvirus renders its encoded GFP an unreliable marker of infection in primary cells. PLoS ONE. 2014;9:e111502 pubmed publisher
Tarrio M, Lee S, Fragoso M, Sun H, Kanno Y, O Shea J, et al. Proliferation conditions promote intrinsic changes in NK cells for an IL-10 response. J Immunol. 2014;193:354-63 pubmed publisher
Flouriot G, Huet G, Demay F, Pakdel F, Boujrad N, Michel D. The actin/MKL1 signalling pathway influences cell growth and gene expression through large-scale chromatin reorganization and histone post-translational modifications. Biochem J. 2014;461:257-68 pubmed publisher
Nikolayeva O, Robinson M. edgeR for differential RNA-seq and ChIP-seq analysis: an application to stem cell biology. Methods Mol Biol. 2014;1150:45-79 pubmed publisher
Goyal A, CHAVEZ S, Reijo Pera R. Generation of human induced pluripotent stem cells using epigenetic regulators reveals a germ cell-like identity in partially reprogrammed colonies. PLoS ONE. 2013;8:e82838 pubmed publisher
Greenberg M, Deleris A, Hale C, Liu A, Feng S, Jacobsen S. Interplay between active chromatin marks and RNA-directed DNA methylation in Arabidopsis thaliana. PLoS Genet. 2013;9:e1003946 pubmed publisher
Shin H, Decotiis J, Giron M, Palmeri D, Lukac D. Histone deacetylase classes I and II regulate Kaposi's sarcoma-associated herpesvirus reactivation. J Virol. 2014;88:1281-92 pubmed publisher
Barrilleaux B, Cotterman R, Knoepfler P. Chromatin immunoprecipitation assays for Myc and N-Myc. Methods Mol Biol. 2013;1012:117-33 pubmed publisher
Lim H, Dimova N, Tan M, Sigoillot F, King R, Shi Y. The G2/M regulator histone demethylase PHF8 is targeted for degradation by the anaphase-promoting complex containing CDC20. Mol Cell Biol. 2013;33:4166-80 pubmed publisher
Lin C, Conti M, Ramalho Santos M. Histone variant H3.3 maintains a decondensed chromatin state essential for mouse preimplantation development. Development. 2013;140:3624-34 pubmed publisher
Leung A, Trac C, Jin W, Lanting L, Akbany A, Sætrom P, et al. Novel long noncoding RNAs are regulated by angiotensin II in vascular smooth muscle cells. Circ Res. 2013;113:266-78 pubmed publisher
Komers R, Mar D, Denisenko O, Xu B, Oyama T, Bomsztyk K. Epigenetic changes in renal genes dysregulated in mouse and rat models of type 1 diabetes. Lab Invest. 2013;93:543-52 pubmed publisher
Malmgren S, Spégel P, Danielsson A, Nagorny C, Andersson L, Nitert M, et al. Coordinate changes in histone modifications, mRNA levels, and metabolite profiles in clonal INS-1 832/13 β-cells accompany functional adaptations to lipotoxicity. J Biol Chem. 2013;288:11973-87 pubmed publisher
Kallsen K, Andresen E, Heine H. Histone deacetylase (HDAC) 1 controls the expression of beta defensin 1 in human lung epithelial cells. PLoS ONE. 2012;7:e50000 pubmed publisher
Shan J, Fu L, Balasubramanian M, Anthony T, Kilberg M. ATF4-dependent regulation of the JMJD3 gene during amino acid deprivation can be rescued in Atf4-deficient cells by inhibition of deacetylation. J Biol Chem. 2012;287:36393-403 pubmed publisher
Demmerle J, Koch A, Holaska J. The nuclear envelope protein emerin binds directly to histone deacetylase 3 (HDAC3) and activates HDAC3 activity. J Biol Chem. 2012;287:22080-8 pubmed publisher
Gupta Agarwal S, Franklin A, Deramus T, Wheelock M, Davis R, McMahon L, et al. G9a/GLP histone lysine dimethyltransferase complex activity in the hippocampus and the entorhinal cortex is required for gene activation and silencing during memory consolidation. J Neurosci. 2012;32:5440-53 pubmed publisher
Thambirajah A, Ng M, Frehlick L, Li A, Serpa J, Petrotchenko E, et al. MeCP2 binds to nucleosome free (linker DNA) regions and to H3K9/H3K27 methylated nucleosomes in the brain. Nucleic Acids Res. 2012;40:2884-97 pubmed publisher
Gagrica S, Brookes S, Anderton E, Rowe J, Peters G. Contrasting behavior of the p18INK4c and p16INK4a tumor suppressors in both replicative and oncogene-induced senescence. Cancer Res. 2012;72:165-75 pubmed publisher
Kondilis Mangum H, Shih H, Mahowald G, Sleckman B, Krangel M. Regulation of TCR? allelic exclusion by gene segment proximity and accessibility. J Immunol. 2011;187:6374-81 pubmed publisher
De la Peña C, Rangel Cano A, Alvarez Venegas R. Regulation of disease-responsive genes mediated by epigenetic factors: interaction of Arabidopsis-Pseudomonas. Mol Plant Pathol. 2012;13:388-98 pubmed publisher
Liu X, Yu C, Duan J, Luo M, Wang K, Tian G, et al. HDA6 directly interacts with DNA methyltransferase MET1 and maintains transposable element silencing in Arabidopsis. Plant Physiol. 2012;158:119-29 pubmed publisher
Wang W, Chen Z, Mao Z, Zhang H, Ding X, Chen S, et al. Nucleolar protein Spindlin1 recognizes H3K4 methylation and stimulates the expression of rRNA genes. EMBO Rep. 2011;12:1160-6 pubmed publisher
Xu C, Cole P, Meyers D, Kormish J, Dent S, Zaret K. Chromatin "prepattern" and histone modifiers in a fate choice for liver and pancreas. Science. 2011;332:963-6 pubmed publisher
Prescott E, Safi A, Rusche L. A region of the nucleosome required for multiple types of transcriptional silencing in Saccharomyces cerevisiae. Genetics. 2011;188:535-48 pubmed publisher
Tchou Wong K, Kiok K, Tang Z, Kluz T, Arita A, Smith P, et al. Effects of nickel treatment on H3K4 trimethylation and gene expression. PLoS ONE. 2011;6:e17728 pubmed publisher
Buzas D, Robertson M, Finnegan E, Helliwell C. Transcription-dependence of histone H3 lysine 27 trimethylation at the Arabidopsis polycomb target gene FLC. Plant J. 2011;65:872-81 pubmed publisher
Calero Nieto F, Wood A, Wilson N, Kinston S, Landry J, Gottgens B. Transcriptional regulation of Elf-1: locus-wide analysis reveals four distinct promoters, a tissue-specific enhancer, control by PU.1 and the importance of Elf-1 downregulation for erythroid maturation. Nucleic Acids Res. 2010;38:6363-74 pubmed publisher
Malik S, Jiang S, Garee J, Verdin E, Lee A, O Malley B, et al. Histone deacetylase 7 and FoxA1 in estrogen-mediated repression of RPRM. Mol Cell Biol. 2010;30:399-412 pubmed publisher
Cotterman R, Knoepfler P. N-Myc regulates expression of pluripotency genes in neuroblastoma including lif, klf2, klf4, and lin28b. PLoS ONE. 2009;4:e5799 pubmed publisher
product information
Catalog Number :
04-745
Subcategory :
Epigenetics & Nuclear Function
Product Name :
Anti-trimethyl-Histone H3 (Lys4) Antibody, clone MC315, rabbit monoclonal
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
Q16695
Host Name :
Rabbit
Antigen :
trimethyl-Histone H3 (Lys4)
Clone :
MC315
Conjugate :
Culture Supernatant
Isotype :
IgG
Product Description :
Anti-trimethyl-Histone H3 (Lys4) Antibody, clone MC315, rabbit monoclonal
Cross Reactivity :
Human
Background :
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
ALT Names :
H3K4me3;Histone H3 (tri methyl K4)
Immunogen :
Peptide containing the sequence [RT trim KQ] in which lysine 4 is trimethylated on human histone H3
Specificity :
Histone H3 containing trimethyl-lysine 4 and, to a lesser extent, dimethyl-lysine 4. See COA for specificity data.
Package Size :
100 µL
Uses :
Western Blotting;Dot Blot;Multiplexing;ChIP-seq
Storage :
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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