Raf-1 Antibody, clone AM223, rabbit monoclonal | EMD Millipore 04-739 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Raf-1 Antibody, clone AM223, rabbit monoclonal

catalog :

04-739

quantity :

100 µL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

AM223

reactivity :

human, mouse

application :

western blot, immunoprecipitation, reverse phase protein lysate microarray, other

citations: 4

Published Application/Species/Sample/Dilution	Reference
other; human; loading ...; fig 4c	Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
reverse phase protein lysate microarray; human; loading ...; fig 3a	. Integrated genomic and molecular characterization of cervical cancer. Nature. 2017;543:378-384 pubmed publisher
	Elster N, Toomey S, Fan Y, Cremona M, Morgan C, Weiner Gorzel K, et al. Frequency, impact and a preclinical study of novel ERBB gene family mutations in HER2-positive breast cancer. Ther Adv Med Oncol. 2018;10:1758835918778297 pubmed publisher
	Baudy A, Dogan T, Flores Mercado J, Hoeflich K, Su F, van Bruggen N, et al. FDG-PET is a good biomarker of both early response and acquired resistance in BRAFV600 mutant melanomas treated with vemurafenib and the MEK inhibitor GDC-0973. EJNMMI Res. 2012;2:22 pubmed publisher

product information

Catalog Number :

04-739

Subcategory :

Cell Signaling

Product Name :

Anti-Raf-1 Antibody, clone AM223, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P04049

Host Name :

Rabbit

Antigen :

Raf-1

Clone :

AM223

Isotype :

IgG

Product Description :

Anti-Raf-1 Antibody, clone AM223, rabbit monoclonal

Cross Reactivity :

Human;Mouse;Rat

Background :

The Raf proteins (Raf-1, A-Raf, B-Raf) are Ser/Thr kinases with homology to the PKC family, containing an N-terminal regulatory domain and a c-terminal catalytic domain. Members of the Raf family bind to activated Ras GTPase, which results in Raf translocation to the plasma membrane and activation. Activated Raf proteins phosphorylate MEKs, and are therefore the principle transducers of signals from Ras to MAP kinase. In addition to its role in mitogenesis, Raf-1 may play a role in regulation of apoptosis and cell cycle progression. Activation of Raf-1 involves phosphorylation of Ser338/339 and Tyr340/341. Activating mutations of B-Raf that disrupt its auto-inhibition loop have been implicated in a number of cancers, including melanoma and colon cancer.

ALT Names :

Oncogene RAF1;raf proto-oncogene serine/threonine protein kinase;v-raf-1 murine leukemia viral oncogene homolog 1

Immunogen :

KLH-conjugated, synthetic peptide corresponding to the C-terminus (amino acids 637-648 (CTLTTSPRLPVF)) of human Raf-1.

Specificity :

Recognizes Raf-1, Mr 74 kDa.

Package Size :

100 µL

Uses :

Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.