phospho-Akt1/PKBα (Ser473) Antibody, clone SK703, rabbit monoclonal

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-Akt1/PKBα (Ser473) Antibody, clone SK703, rabbit monoclonal

catalog :

04-736

quantity :

100 μl

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SK703

reactivity :

human, mouse, chicken, bovine

application :

western blot

citations: 3

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 2	Vallo S, Michaelis M, Gust K, Black P, Rothweiler F, Kvasnicka H, et al. Dasatinib enhances tumor growth in gemcitabine-resistant orthotopic bladder cancer xenografts. BMC Res Notes. 2016;9:454 pubmed
	Moore C, Palau V, Mahboob R, Lightner J, Stone W, Krishnan K. Upregulation of pERK and c-JUN by γ-tocotrienol and not α-tocopherol are essential to the differential effect on apoptosis in prostate cancer cells. BMC Cancer. 2020;20:428 pubmed publisher
	Loi M, Becherini P, Emionite L, Giacomini A, Cossu I, Destefanis E, et al. sTRAIL coupled to liposomes improves its pharmacokinetic profile and overcomes neuroblastoma tumour resistance in combination with Bortezomib. J Control Release. 2014;192:157-66 pubmed publisher

product information

Catalog Number :

04-736

Subcategory :

Cell Signaling

Product Name :

Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone SK703, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P31749

Host Name :

Rabbit

Antigen :

phospho-Akt1/PKBα (Ser473)

Clone :

SK703

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone SK703, rabbit monoclonal

Cross Reactivity :

Human;Mouse

Background :

The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKBα or RacPK), Akt2 (also designated PKB-β or RacPK-β) and Akt3 (also designated PKB-γ or thyoma viral proto-oncogene 3). Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-β tyrosine residues 740 and 751, which bind the 85 kDa subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1 (IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1- stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin. Taken together, this data strongly suggests that the protein signals downstream of the PI kinases.

ALT Names :

AKT1 kinase;Protein kinase B;RAC-alpha serine/threonine-protein kinase ;rac protein kinase alpha ;v-akt murine thymoma viral oncogene homolog 1

Immunogen :

Peptide containing a pSer that corresponds to amino acid position 473 of human Akt1/PKBa

Specificity :

Recognizes phosphorylated Akt1/PKBα, Mr 60 kDa. Cross-reactivity to phosphorylated Akt2 and Akt3 likely based on sequence homology.

Package Size :

100 μl

Uses :

Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.