monomethyl-Histone H4 (Lys20) Antibody, clone NL314, rabbit monoclonal

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

monomethyl-Histone H4 (Lys20) Antibody, clone NL314, rabbit monoclonal

catalog :

04-735

quantity :

100 µl

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

NL314

reactivity :

human

application :

western blot

citations: 3

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 6	Nelson D, Jaber Hijazi F, Cole J, Robertson N, Pawlikowski J, Norris K, et al. Mapping H4K20me3 onto the chromatin landscape of senescent cells indicates a function in control of cell senescence and tumor suppression through preservation of genetic and epigenetic stability. Genome Biol. 2016;17:158 pubmed publisher
western blot; human; loading ...; fig 7b	Rizzardi L, Coleman K, Varma D, Matson J, Oh S, Cook J. CDK1-dependent inhibition of the E3 ubiquitin ligase CRL4CDT2 ensures robust transition from S Phase to Mitosis. J Biol Chem. 2015;290:556-67 pubmed publisher
	Edwards C, Dang W, Berger S. Histone H4 lysine 20 of Saccharomyces cerevisiae is monomethylated and functions in subtelomeric silencing. Biochemistry. 2011;50:10473-83 pubmed publisher

product information

Catalog Number :

04-735

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-monomethyl-Histone H4 (Lys20) Antibody, clone NL314, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P62805

Host Name :

Rabbit

Antigen :

monomethyl-Histone H4 (Lys20)

Clone :

NL314

Conjugate :

Culture Supernatant

Isotype :

IgG

Product Description :

Anti-monomethyl-Histone H4 (Lys20) Antibody, clone NL314, rabbit monoclonal

Cross Reactivity :

Human

Background :

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications, which include acetylation, phosphorylation, methylation, and ubiquitin-ation, occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Trimethylation of histone H4 on lys20 (H4-K20me3) selectively marks constitutive pericentromeric hetero-chromatin and imprinting control regions, while mono- and dimethylation are broadly distributed but principally enriched in euchromatin regions. Although the biological function of H4K20me remains poorly understood, studies suggest that H4-K20me2 is involved in the guidance of DNA repair proteins to DNA strand breaks, whereas H4K20me1 is associated with chromatin condensation processes. PR-Set7 (also known as SET8) specifically catalyzes monomethylation of H4K20me1.

ALT Names :

H4K20me1;Histone H4 (mono methyl K20);histone 4, H4;histone H4;histone cluster 4, H4

Immunogen :

KLH-conjugated, synthetic peptide containing the sequence …HR[mmK]V…, in which mmK corresponds to monomethyl-lysine at residue 20 of human histone H4.

Specificity :

Recognizes monomethyl-histone H4 (Lys20), Mr ~10kDa.

Package Size :

100 µl

Uses :

Western Blotting;Multiplexing

Storage :

Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.