Dicer1 Antibody, clone 5D12.2 | EMD Millipore 04-721 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Dicer1 Antibody, clone 5D12.2

catalog :

04-721

quantity :

100 μL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5D12.2

reactivity :

human

application :

western blot

citations: 3

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:5000	Shi J, Duan Z, Sun J, Wu M, Wang B, Zhang J, et al. Identification and validation of a novel microRNA-like molecule derived from a cytoplasmic RNA virus antigenome by bioinformatics and experimental approaches. Virol J. 2014;11:121 pubmed publisher
	Liang H, Zhou Z, Zhang S, Zen K, Chen X, Zhang C. Identification of Ebola virus microRNAs and their putative pathological function. Sci China Life Sci. 2014;57:973-81 pubmed publisher
	Shi J, Sun J, Wang B, Wu M, Zhang J, Duan Z, et al. Novel microRNA-like viral small regulatory RNAs arising during human hepatitis A virus infection. FASEB J. 2014;28:4381-93 pubmed publisher

product information

Catalog Number :

04-721

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Dicer1 Antibody, clone 5D12.2

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q9UPY3

Host Name :

Mouse

Antigen :

Dicer1

Clone :

5D12.2

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-Dicer1 Antibody, clone 5D12.2

Cross Reactivity :

Human

Background :

RNA interference (RNAi) is mechanism for post-transcriptional gene silencing that leads to the loss of the activity of the gene. RNAi is activated when long dsRNAs are cleaved by the enzyme Dicer1 (RNase III family of dsRNA-specific endonucleases) into 21-23 nucleotide siRNA (small interfering RNA). These siRNAs bind proteins to form the RISC (RNA-induced silencing complex) which is activated when the double stranded siRNAs are unzipped to single stranded siRNAs. Activated RISC recognizes and binds complementary sequences in mRNA. The mRNA is cleaved and the overall effect is an inhibition of the protein expression and a loss of the gene activity. Dicer1 along with the Argonaute family of proteins (Ago1 and Ago2) share the same domain PIWI and PAZ domain that provide endonuclease activity to RNA-induced silencing complexes (RISC).

ALT Names :

DCR1;Dicer;HERNA;KIAA0928

Immunogen :

KLH conjugated synthetic peptide corresponding to amino acids 378-385 of human Dicer1

Specificity :

Human Dicer1

Package Size :

100 μL

Uses :

Western Blotting

Storage :

Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.