Ago2 Antibody, clone 9E8.2 | EMD Millipore 04-642 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Ago2 Antibody, clone 9E8.2

catalog :

04-642

quantity :

100 μL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

9E8.2

reactivity :

human, mouse

application :

western blot

citations: 5

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:2000; fig 6	Pandolfini L, Luzi E, Bressan D, Ucciferri N, Bertacchi M, Brandi R, et al. RISC-mediated control of selected chromatin regulators stabilizes ground state pluripotency of mouse embryonic stem cells. Genome Biol. 2016;17:94 pubmed publisher
	Smith K, Starmer J, Miller S, Sethupathy P, Magnuson T. Long Noncoding RNA Moderates MicroRNA Activity to Maintain Self-Renewal in Embryonic Stem Cells. Stem Cell Reports. 2017;9:108-121 pubmed publisher
	Shang R, Zhang F, Xu B, Xi H, Zhang X, Wang W, et al. Ribozyme-enhanced single-stranded Ago2-processed interfering RNA triggers efficient gene silencing with fewer off-target effects. Nat Commun. 2015;6:8430 pubmed publisher
	Heinrich E, Wagner J, Kruger M, John D, Uchida S, Weigand J, et al. Regulation of miR-17-92a cluster processing by the microRNA binding protein SND1. FEBS Lett. 2013;587:2405-11 pubmed publisher
	Hafner M, Lianoglou S, Tuschl T, Betel D. Genome-wide identification of miRNA targets by PAR-CLIP. Methods. 2012;58:94-105 pubmed publisher

product information

Catalog Number :

04-642

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Ago2 Antibody, clone 9E8.2

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q9UKV8

Host Name :

Mouse

Antigen :

Ago2

Clone :

9E8.2

Conjugate :

Ascites

Isotype :

IgG1κ

Product Description :

Anti-Ago2 Antibody, clone 9E8.2

Cross Reactivity :

Human

Background :

Ago2 (argonaute-2), also known as eukaryotic translation initiation factor 2C (EIF2C2), is an essential component for siRNA-directed RNA interference (RNAi) response. Ago2 is an endonuclease required for the unwinding of siRNA duplex and assembly of siRNA into RNA-induced silencing complexes (RISC). Ago2 interacts with DICER1 through its Piwi domain. This Piwi domain is thought to provide RNA cleavage activity via a mechanism similar to RNase H. Ago2 activity is necessary for embryonic development as well as RNA-mediated gene silencing (RNAi).

ALT Names :

Argonaute-2;Eukaryotic translation initiation factor 2C;AGO2;eIF-2C;Slicer protein;MGC3183;Piwi domain protein;PPD

Immunogen :

KLH-conjugated, synthetic peptide containing the sequence YSGAGPALAPPAPPPPIQG

Specificity :

human Ago2

Package Size :

100 μL

Uses :

Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance