PTEN rabbit monoclonal Antibody | EMD Millipore 04-409 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

PTEN rabbit monoclonal Antibody

catalog :

04-409

quantity :

100 µL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; mouse western blot; human; fig 5	Benoit Y, Witherspoon M, Laursen K, Guezguez A, Beauséjour M, Beaulieu J, et al. Pharmacological inhibition of polycomb repressive complex-2 activity induces apoptosis in human colon cancer stem cells. Exp Cell Res. 2013;319:1463-70 pubmed publisher
	Spitzenberg V, König C, Ulm S, Marone R, Röpke L, Müller J, et al. Targeting PI3K in neuroblastoma. J Cancer Res Clin Oncol. 2010;136:1881-90 pubmed publisher

product information

Catalog Number :

04-409

Subcategory :

Cell Signaling

Product Name :

Anti-PTEN rabbit monoclonal Antibody

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P60484

Host Name :

Rabbit

Antigen :

PTEN rabbit monoclonal

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-PTEN rabbit monoclonal Antibody

Cross Reactivity :

Human;Mouse;Rat

Background :

PTEN acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine- phosphorylated proteins. It also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3- phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 PtdIns(3,4)P2 PtdIns3P Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. PTEN antagonizes the PI3K- AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. It dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. PTEN may be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. Recent research shows a role for PTEN in addiction.

ALT Names :

MMAC1 phosphatase and tensin homolog deleted on chromosome 10;Mutated in multiple advanced cancers 1;phosphatase and tensin homolog;phosphatase and tensin homolog (mutated in multiple advanced cancers 1).

Immunogen :

KLH-conjugated synthetic peptide corresponding to the C-terminal region of human PTEN.

Specificity :

This antibody recognizes the C-terminal domain of PTEN.

Package Size :

100 µL

Uses :

Flow Cytometry;Immunofluorescence;Immunohistochemistry;Immunohistochemistry (Paraffin);Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.