PI3 Kinase Antibody, p85α rabbit monoclonal | EMD Millipore 04-403 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

PI3 Kinase Antibody, p85α rabbit monoclonal

catalog :

04-403

quantity :

100 µL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

citations: 5

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse western blot; mouse	Kapodistria K, Tsilibary E, Politis P, Moustardas P, Charonis A, Kitsiou P. Nephrin, a transmembrane protein, is involved in pancreatic beta-cell survival signaling. Mol Cell Endocrinol. 2015;400:112-28 pubmed publisher
	Kim N, Gumbiner B. Adhesion to fibronectin regulates Hippo signaling via the FAK-Src-PI3K pathway. J Cell Biol. 2015;210:503-15 pubmed publisher
	Chen F, Chen J, Lin J, Cheltsov A, Xu L, Chen Y, et al. NSC-640358 acts as RXRÎ± ligand to promote TNFÎ±-mediated apoptosis of cancer cell. Protein Cell. 2015;6:654-666 pubmed publisher
	Sohn W, Gwon G, An C, Moon C, Bae Y, Yamamoto H, et al. Morphological evidences in circumvallate papilla and von Ebners' gland development in mice. Anat Cell Biol. 2011;44:274-83 pubmed publisher
	Messmer Blust A, Balasubramanian S, Gorbacheva V, Jeyaratnam J, Vestal D. The interferon-gamma-induced murine guanylate-binding protein-2 inhibits rac activation during cell spreading on fibronectin and after platelet-derived growth factor treatment: role for phosphatidylinositol 3-kinase. Mol Biol Cell. 2010;21:2514-28 pubmed publisher

product information

Catalog Number :

04-403

Subcategory :

Cell Signaling

Product Name :

Anti-PI3 Kinase Antibody, p85α rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P27986

Host Name :

Rabbit

Antigen :

PI3 Kinase

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-PI3 Kinase Antibody, p85α rabbit monoclonal

Cross Reactivity :

Human;Mouse;Rat

Background :

PI3 Kinase (Phosphatidylinositol 3-Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospho-lipid substrate on the membrane. PI3 Kinase is also activated by Ras, and by the β:γ subunits of heterotrimeric G-proteins. PI3 Kinase is inhibitable by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway.

ALT Names :

PI3-kinase p85 subunit alpha;PtdIns-3-kinase p85-alpha;phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha);phosphatidylinositol 3-kinase, regulatory, 1;phosphatidylinositol 3-kinase-associated p-85 alpha;phosphoinositide-3-kinase, regulatory subunit 1 (alpha);phosphoinositide-3-kinase, regulatory subunit 1 (p85 alpha);phosphoinositide-3-kinase, regulatory subunit, polypeptide 1 (p85 alpha).

Immunogen :

KLH-conjugated synthetic peptide corresponding to the C-terminal region of human PI3 Kinase, p85α subunit.

Specificity :

This antibody recognizes the C-terminal domain of PI3 Kinase, p85α subunit.

Package Size :

100 µL

Uses :

Flow Cytometry;Immunocytochemistry;Immunofluorescence;Immunohistochemistry (Paraffin);Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.