PI3 Kinase Antibody, p110β, rabbit monoclonal | EMD Millipore 04-400 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

PI3 Kinase Antibody, p110β, rabbit monoclonal

catalog :

04-400

quantity :

100 µL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

Y384

The same clone is also sold as:

Abcam: ab32569

reactivity :

human

application :

western blot, immunocytochemistry, immunoprecipitation, flow cytometry

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; fig st1	Treindl F, Ruprecht B, Beiter Y, Schultz S, Döttinger A, Staebler A, et al. A bead-based western for high-throughput cellular signal transduction analyses. Nat Commun. 2016;7:12852 pubmed publisher
	Spitzenberg V, König C, Ulm S, Marone R, Röpke L, Müller J, et al. Targeting PI3K in neuroblastoma. J Cancer Res Clin Oncol. 2010;136:1881-90 pubmed publisher

product information

Catalog Number :

04-400

Subcategory :

Cell Signaling

Product Name :

Anti-PI3 Kinase Antibody, p110β, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P42338

Host Name :

Rabbit

Antigen :

PI3 Kinase

Clone :

Y384

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-PI3 Kinase Antibody, p110β, rabbit monoclonal

Cross Reactivity :

Human

Background :

PI3 Kinase (Phosphatidylinositol 3-Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospho-lipid substrate on the membrane. PI3 Kinase is also activated by Ras, and by the β:γ subunits of heterotrimeric G-proteins. PI3 Kinase is inhibitable by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway.

ALT Names :

PI3-kinase p110 subunit beta;PtdIns-3-kinase p110;phosphatidylinositol 3-kinase, catalytic, beta polypeptide;phosphoinositide-3-kinase, catalytic, beta polypeptide

Immunogen :

KLH-conjugated synthetic peptide corresponding to the N-terminal region of human PI3 Kinase, p110β

Specificity :

This antibody recognizes a region in the N-terminus domain of human PI3 Kinase, p110β. Non specific band detected at MW 55 kDa.

Package Size :

100 µL

Uses :

Flow Cytometry;Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage