mTOR Antibody, rabbit monoclonal | EMD Millipore 04-385 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

mTOR Antibody, rabbit monoclonal

catalog :

04-385

quantity :

100 µL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

citations: 10

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; loading ...; fig 6h western blot; mouse; 1:1000; loading ...; fig 6h	Xu Y, Wang Y, Yao A, Xu Z, Dou H, Shen S, et al. Low Frequency Magnetic Fields Induce Autophagy-associated Cell Death in Lung Cancer through miR-486-mediated Inhibition of Akt/mTOR Signaling Pathway. Sci Rep. 2017;7:11776 pubmed publisher
western blot; rat; 1:1000; fig 2e	Chen K, Tsai M, Wu C, Jou M, Wei I, Huang C. AMPA Receptor-mTOR Activation is Required for the Antidepressant-Like Effects of Sarcosine during the Forced Swim Test in Rats: Insertion of AMPA Receptor may Play a Role. Front Behav Neurosci. 2015;9:162 pubmed publisher
western blot; rat	Pantazi E, Zaouali M, Bejaoui M, Folch Puy E, Ben Abdennebi H, Varela A, et al. Sirtuin 1 in rat orthotopic liver transplantation: an IGL-1 preservation solution approach. World J Gastroenterol. 2015;21:1765-74 pubmed publisher
western blot; human	Liu J, Zheng L, Ma L, Wang B, Zhao Y, Wu N, et al. Oleanolic acid inhibits proliferation and invasiveness of Kras-transformed cells via autophagy. J Nutr Biochem. 2014;25:1154-1160 pubmed publisher
immunohistochemistry - paraffin section; rat; 1:250	Sereno J, Nunes S, Rodrigues Santos P, Vala H, Rocha Pereira P, Fernandes J, et al. Conversion to sirolimus ameliorates cyclosporine-induced nephropathy in the rat: focus on serum, urine, gene, and protein renal expression biomarkers. Biomed Res Int. 2014;2014:576929 pubmed publisher
	Liu Y, Li L, An S, Zhang Y, Feng S, Zhao L, et al. Antifatigue Effects of Antrodia cinnamomea Cultured Mycelium via Modulation of Oxidative Stress Signaling in a Mouse Model. Biomed Res Int. 2017;2017:9374026 pubmed publisher
	Zhao M, Luo R, Liu Y, Gao L, Fu Z, Fu Q, et al. miR-3188 regulates nasopharyngeal carcinoma proliferation and chemosensitivity through a FOXO1-modulated positive feedback loop with mTOR-p-PI3K/AKT-c-JUN. Nat Commun. 2016;7:11309 pubmed publisher
	Chen S, Liu J, Wang X, Liang C, Ding J, Meng L. Inhibition of tumor cell growth, proliferation and migration by X-387, a novel active-site inhibitor of mTOR. Biochem Pharmacol. 2012;83:1183-94 pubmed publisher
	Van Aller G, Carson J, Tang W, Peng H, Zhao L, Copeland R, et al. Epigallocatechin gallate (EGCG), a major component of green tea, is a dual phosphoinositide-3-kinase/mTOR inhibitor. Biochem Biophys Res Commun. 2011;406:194-9 pubmed publisher
	Cohet N, Stewart K, Mudhasani R, Asirvatham A, Mallappa C, Imbalzano K, et al. SWI/SNF chromatin remodeling enzyme ATPases promote cell proliferation in normal mammary epithelial cells. J Cell Physiol. 2010;223:667-78 pubmed publisher

product information

Catalog Number :

04-385

Subcategory :

Cell Signaling

Product Name :

Anti-mTOR Antibody, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P42345

Host Name :

Rabbit

Antigen :

mTOR

Conjugate :

Culture Supernatant

Product Description :

Anti-mTOR Antibody, rabbit monoclonal

Cross Reactivity :

Human;Mouse;Rat

Background :

mTOR (Mammalian Target of Rapamycin, aka FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. Interestingly, mTOR is the central component of two multimeric kinase complexes consisting of mTOR and numerous other mTOR binding proteins. These two multimeric protein complexes are designated mTORC1 and mTORC2. mTORC1 (mTOR Complex 1) consists of at least mTOR, Raptor, and GL (mLST8). mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. The complex is activated primarily though the PI3 Kinase/Akt pathway. Upon insulin stimulation, Akt activates mTORC1 by phosphorylating and inhibiting TSC (and possibly other yet discovered targets and/or mTOR itself). This inhibits the upstream small GTPase regulator Rheb (Ras homolog enriched in brain). This inhibits the kinase activity of the mTORC1 complex, thus disabling its ability to phosphorylate its downstream targets such as p70 S6K on Thr389 and 4E-BP1 on Thr229. The other mTOR complex, mTORC2 (mTOR Complex 2), is made up of at least mTOR, Rictor, GL, Sin1, Protor 1 and 2. mTORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473, a well-known effecter of the PI3 Kinase pathway. In addition to phosphorylating Akt, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Interestingly, unlike mTORC1, the mTORC2 complex appears to not be inhibited by treatment with rapamycin and for this reason is referred to as the rapamycin-insensitive complex. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.

ALT Names :

FRAP1, FRAP, FRAP2, RAFT1, RAPT1, FK506 binding protein 12-rapamycin associated protein 1

Immunogen :

KLH-conjugated synthetic peptide corresponding to the C-terminal region of human mTOR.

Specificity :

Recognizes a region in the C-terminus domain of human mTOR.

Package Size :

100 µL

Uses :

Flow Cytometry;Immunocytochemistry;Immunohistochemistry (Paraffin);Immunoprecipitation;Western Blotting

Storage :

Stable for 2 years at -20°C from date of shipment.