IKK alpha Antibody, clone Y463,, rabbit monoclonal | EMD Millipore 04-365 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

IKK alpha Antibody, clone Y463,, rabbit monoclonal

catalog :

04-365

quantity :

100 µL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

Y463

The same clone is also sold as:

Abcam: ab169743, ab32041

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

product information

Catalog Number :

04-365

Subcategory :

Cell Signaling

Product Name :

Anti-IKK alpha Antibody, clone Y463,, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

O15111

Host Name :

Rabbit

Antigen :

IKK alpha

Clone :

Y463

Conjugate :

Unpurified

Isotype :

IgG

Product Description :

Anti-IKK alpha Antibody, clone Y463,, rabbit monoclonal

Cross Reactivity :

Human;Mouse;Rat

Background :

IKK-alpha (I-Kappa-B kinase-alpha) is a member of the IKK complex which is composed of IKK-alpha, IKK-beta, IKK-gamma and IKAP (1-2). Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. Activation of NFKB by TNF requires phosphorylation of IKKalpha at threonine 23 and serine 176 by AKT1 and NIK respectively (3). It has been demonstrated that IKK-alpha can accumulate in the nucleus after cytokine exposure (4). In the nucleus IKK-alpha interacts with CREB-binding protein in conjunction with RELA to be recruited to the NF-kappa-B-responsive promoters in order to mediate the cytokine-induced phosphorylation and subsequent acetylation of specific residues in histone H3 (5).

ALT Names :

conserved helix-loop-helix ubiquitous kinase;IKK-alpha;Transcription factor 16;I-kappa-B kinase 1;I-kappa-B kinase-alpha;IkB kinase alpha subunit;IKK-a kinase;inhibitor of nuclear factor kappa-B kinase subunit alpha;Nuclear factor NFkappaB inhibitor kinase alpha;I-kappa-B kinase alpha;IkappaB kinase;Nuclear factor NF-kappa-B inhibitor kinase alpha

Immunogen :

Synthetic peptide corresponding to the N-terminus of human IKK-alpha.

Package Size :

100 µL

Uses :

Western Blotting;Immunocytochemistry;Immunoprecipitation;Flow Cytometry;Immunohistochemistry (Paraffin)

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.