EGFR Antibody, clone E114, rabbit monoclonal | EMD Millipore 04-337 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

EGFR Antibody, clone E114, rabbit monoclonal

catalog :

04-337

quantity :

100 µL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

E114

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

product information

Catalog Number :

04-337

Subcategory :

Cell Signaling

Product Name :

Anti-EGFR Antibody, clone E114, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P00533

Host Name :

Rabbit

Antigen :

EGFR

Clone :

E114

Conjugate :

Unpurified

Isotype :

IgG

Product Description :

Anti-EGFR Antibody, clone E114, rabbit monoclonal

Cross Reactivity :

Human;Mouse;Rat

Background :

Epidermal growth factor receptor (EGFR) is a 170 kDa transmembrane glycoprotein receptor tyrosine kinase that, activated by epidermal growth factor (EGF), affects cell growth and differentiation (1). Binding of EGF or TGF alpha to EGFR activates tyrosine kinase activity of the receptor (2). Phosphorylation of Tyr 992, Tyr 1068 and Tyr 1086 is required for conformational change in the C-terminal of EGFR (3). Autophosphorylation of Tyr 992 creates a binding site for the phospholipase C-gamma (PLCgamma) SH2 domain, inducing downstream signaling (4).

ALT Names :

epidermal growth factor receptor;Proto-oncogene c-ErbB-1;Receptor tyrosine-protein kinase erbB-1;epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b) oncogene homolog);avian erythroblastic leukemia viral (v-erb-b) oncogene homolog;cell growth inhibiting protein 40;cell proliferation-inducing protein 61

Immunogen :

Synthetic peptide corresponding to the C-terminus of human EGFR.

Package Size :

100 µL

Uses :

Western Blotting;Immunocytochemistry;Immunoprecipitation;Immunohistochemistry

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.