This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Ubiquitinylated proteins Antibody, clone FK2
catalog :
04-263
quantity :
100 µL
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
FK2
reactivity :
human, mouse, rat, roundworm
application :
western blot, ELISA, immunocytochemistry, immunoprecipitation, other
citations: 26
Published Application/Species/Sample/DilutionReference
  • other; mouse; 1:400; loading ...; fig 5
Budzik J, Swaney D, Jimenez Morales D, Johnson J, Garelis N, Repasy T, et al. Dynamic post-translational modification profiling of Mycobacterium tuberculosis-infected primary macrophages. elife. 2020;9: pubmed publisher
  • immunocytochemistry; human; loading ...; fig 4g
Jachimowicz R, Beleggia F, Isensee J, Velpula B, Goergens J, Bustos M, et al. UBQLN4 Represses Homologous Recombination and Is Overexpressed in Aggressive Tumors. Cell. 2019;176:505-519.e22 pubmed publisher
  • western blot; rat; 1:2000; loading ...; fig 2a
Xu J, Kurup P, Nairn A, Lombroso P. Synaptic NMDA Receptor Activation Induces Ubiquitination and Degradation of STEP61. Mol Neurobiol. 2018;55:3096-3111 pubmed publisher
  • western blot; human; loading ...; fig 2a
To M, Peterson C, Roberts M, Counihan J, Wu T, Forster M, et al. Lipid disequilibrium disrupts ER proteostasis by impairing ERAD substrate glycan trimming and dislocation. Mol Biol Cell. 2017;28:270-284 pubmed publisher
  • immunocytochemistry; roundworm ; 1:100; loading ...; fig 3a
Ackermann L, Schell M, Pokrzywa W, Kevei Ã, Gartner A, Schumacher B, et al. E4 ligase-specific ubiquitination hubs coordinate DNA double-strand-break repair and apoptosis. Nat Struct Mol Biol. 2016;23:995-1002 pubmed publisher
  • western blot; mouse; loading ...; fig 7d
Wu Y, Ren D, Chen G. Siglec-E Negatively Regulates the Activation of TLR4 by Controlling Its Endocytosis. J Immunol. 2016;197:3336-3347 pubmed
  • immunoprecipitation; mouse; 1:100; loading ...; fig s4b
Naik E, Dixit V. Usp9X Is Required for Lymphocyte Activation and Homeostasis through Its Control of ZAP70 Ubiquitination and PKCβ Kinase Activity. J Immunol. 2016;196:3438-51 pubmed publisher
  • western blot; human; fig 6b
Zhang P, Feng S, Liu G, Wang H, Zhu H, Ren Q, et al. Mutant B-Raf(V600E) Promotes Melanoma Paracellular Transmigration by Inducing Thrombin-mediated Endothelial Junction Breakdown. J Biol Chem. 2016;291:2087-106 pubmed publisher
  • immunocytochemistry; human; fig s3
Fuchs M, Luthold C, Guilbert S, Varlet A, Lambert H, Jetté A, et al. A Role for the Chaperone Complex BAG3-HSPB8 in Actin Dynamics, Spindle Orientation and Proper Chromosome Segregation during Mitosis. PLoS Genet. 2015;11:e1005582 pubmed publisher
  • immunocytochemistry; human; fig 5b
Selleck E, Orchard R, Lassen K, Beatty W, Xavier R, Levine B, et al. A Noncanonical Autophagy Pathway Restricts Toxoplasma gondii Growth in a Strain-Specific Manner in IFN-γ-Activated Human Cells. MBio. 2015;6:e01157-15 pubmed publisher
  • western blot; human; fig 3d
Drießen S, Berleth N, Friesen O, Löffler A, Böhler P, Hieke N, et al. Deubiquitinase inhibition by WP1130 leads to ULK1 aggregation and blockade of autophagy. Autophagy. 2015;11:1458-70 pubmed publisher
  • western blot; human; fig 7
Cha Molstad H, Sung K, Hwang J, Kim K, Yu J, Yoo Y, et al. Amino-terminal arginylation targets endoplasmic reticulum chaperone BiP for autophagy through p62 binding. Nat Cell Biol. 2015;17:917-29 pubmed publisher
  • western blot; human; 1:1,000
Medina F, Quintremil S, Alberti C, Barriga A, Cartier L, Puente J, et al. Tax posttranslational modifications and interaction with calreticulin in MT-2 cells and human peripheral blood mononuclear cells of human T cell lymphotropic virus type-I-associated myelopathy/tropical spastic paraparesis patients. AIDS Res Hum Retroviruses. 2014;30:370-9 pubmed publisher
Seo H, Choi Y, Kim M, Kang K, Urano T, Lee D. The 19S proteasome is directly involved in the regulation of heterochromatin spreading in fission yeast. J Biol Chem. 2017;292:17144-17155 pubmed publisher
Li Y, Luo K, Yin Y, Wu C, Deng M, Li L, et al. USP13 regulates the RAP80-BRCA1 complex dependent DNA damage response. Nat Commun. 2017;8:15752 pubmed publisher
An L, Jiang Y, Ng H, Man E, Chen J, Khoo U, et al. Dual-utility NLS drives RNF169-dependent DNA damage responses. Proc Natl Acad Sci U S A. 2017;114:E2872-E2881 pubmed publisher
Malecki J, Aileni V, Ho A, Schwarz J, Moen A, Sørensen V, et al. The novel lysine specific methyltransferase METTL21B affects mRNA translation through inducible and dynamic methylation of Lys-165 in human eukaryotic elongation factor 1 alpha (eEF1A). Nucleic Acids Res. 2017;45:4370-4389 pubmed publisher
Milan G, Romanello V, Pescatore F, Armani A, Paik J, Frasson L, et al. Regulation of autophagy and the ubiquitin-proteasome system by the FoxO transcriptional network during muscle atrophy. Nat Commun. 2015;6:6670 pubmed publisher
Vural A, McQuiston T, Blumer J, Park C, Hwang I, Williams Bey Y, et al. Normal autophagic activity in macrophages from mice lacking G?i3, AGS3, or RGS19. PLoS ONE. 2013;8:e81886 pubmed publisher
Hunt L, Demontis F. Whole-mount immunostaining of Drosophila skeletal muscle. Nat Protoc. 2013;8:2496-501 pubmed publisher
Viñas Castells R, Frias A, Robles Lanuza E, Zhang K, Longmore G, Garcia de Herreros A, et al. Nuclear ubiquitination by FBXL5 modulates Snail1 DNA binding and stability. Nucleic Acids Res. 2014;42:1079-94 pubmed publisher
Okamoto K, Bartocci C, Ouzounov I, Diedrich J, Yates J, Denchi E. A two-step mechanism for TRF2-mediated chromosome-end protection. Nature. 2013;494:502-5 pubmed publisher
Kirchner P, Bug M, Meyer H. Ubiquitination of the N-terminal region of caveolin-1 regulates endosomal sorting by the VCP/p97 AAA-ATPase. J Biol Chem. 2013;288:7363-72 pubmed publisher
Leung J, Wang Y, Fong K, Huen M, Li L, Chen J. Fanconi anemia (FA) binding protein FAAP20 stabilizes FA complementation group A (FANCA) and participates in interstrand cross-link repair. Proc Natl Acad Sci U S A. 2012;109:4491-6 pubmed publisher
Le Goff C, Mahaut C, Abhyankar A, Le Goff W, Serre V, Afenjar A, et al. Mutations at a single codon in Mad homology 2 domain of SMAD4 cause Myhre syndrome. Nat Genet. 2011;44:85-8 pubmed publisher
Kovacs J, Li C, Yang Q, Li G, Garcia I, Ju S, et al. Autophagy promotes T-cell survival through degradation of proteins of the cell death machinery. Cell Death Differ. 2012;19:144-52 pubmed publisher
product information
Catalog Number :
04-263
Subcategory :
Protein Trafficking
Product Name :
Anti-Ubiquitinylated proteins Antibody, clone FK2
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Host Name :
Mouse
Antigen :
Ubiquitinylated proteins
Conjugate :
Purified
Isotype :
IgG1
Product Description :
Anti-Ubiquitinylated proteins Antibody, clone FK2
Cross Reactivity :
All
Immunogen :
Poly-ubiquitinylated-lysozyme.
Specificity :
Recognizes mono- and poly ubiquitinylated proteins, but not free ubiquitin.
Package Size :
100 µL
Uses :
ELISA;Immunofluorescence;Immunoprecipitation;Western Blotting
Storage :
Stable for 1 year at -20°C from date of shipment for up to 12 months.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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