p27(Kip1) Antibody, clone Y236, rabbit monoclonal | EMD Millipore 04-240 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

p27(Kip1) Antibody, clone Y236, rabbit monoclonal

catalog :

04-240

quantity :

100 µL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

Y236

The same clone is also sold as:

Abcam: ab32034

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

citations: 5

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 1c	Randles L, Anchoori R, Roden R, Walters K. The Proteasome Ubiquitin Receptor hRpn13 and Its Interacting Deubiquitinating Enzyme Uch37 Are Required for Proper Cell Cycle Progression. J Biol Chem. 2016;291:8773-83 pubmed publisher
	Zhang L, Krausz T, DeMay R. A Pilot Study of Galectin-3, HBME-1, and p27 Triple Immunostaining Pattern for Diagnosis of Indeterminate Thyroid Nodules in Cytology With Correlation to Histology. Appl Immunohistochem Mol Morphol. 2015;23:481-90 pubmed publisher
	Zhang L, DeMay R. Cytological features of mixed adenoneuroendocrine carcinoma of the ampulla: two case reports with review of literature. Diagn Cytopathol. 2014;42:1075-84 pubmed publisher
	Wan X, Zhao Y, Fan X, Cai H, Zhang Y, Chen M, et al. Molecular prognostic prediction for locally advanced nasopharyngeal carcinoma by support vector machine integrated approach. PLoS ONE. 2012;7:e31989 pubmed publisher
	Jeanblanc M, Ragu S, Gey C, Contrepois K, Courbeyrette R, Thuret J, et al. Parallel pathways in RAF-induced senescence and conditions for its reversion. Oncogene. 2012;31:3072-85 pubmed publisher

product information

Catalog Number :

04-240

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-p27(Kip1) Antibody, clone Y236, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P46527

Host Name :

Rabbit

Antigen :

p27(Kip1)

Clone :

Y236

Conjugate :

Culture Supernatant

Isotype :

IgG

Product Description :

Anti-p27(Kip1) Antibody, clone Y236, rabbit monoclonal

Cross Reactivity :

Human;Rat

Background :

p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4. p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1); while phosphorylation by hKIS on Serine 10 signals the nuclear export to the cytoplasm.

ALT Names :

Cyclin-dependent kinase inhibitor ;Cyclin-dependent kinase inhibitor p27 ;cyclin-dependent kinase inhibitor 1B;cyclin-dependent kinase inhibitor 1B (p27, Kip1)

Immunogen :

Synthetic peptide corresponding to the C-terminus of human p27 (Kip1).

Specificity :

This antibody recognizes p27(Kip1) at the C-terminus.

Package Size :

100 µL

Uses :

Western Blotting;Flow Cytometry;Immunoprecipitation;Immunocytochemistry;Immunohistochemistry

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.