NFĸB Antibody, p65, clone E379, rabbit monoclonal | EMD Millipore 04-235 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

NFĸB Antibody, p65, clone E379, rabbit monoclonal

catalog :

04-235

quantity :

100 μL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

E379

The same clone is also sold as:

Abcam: ab190589, ab208750, ab32536

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

citations: 2

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:100; fig 8	Sung S, Wu I, Chuang P, Petros J, Wu H, Zeng H, et al. Targeting L1 cell adhesion molecule expression using liposome-encapsulated siRNA suppresses prostate cancer bone metastasis and growth. Oncotarget. 2014;5:9911-29 pubmed
	Srivastava T, Sharma M, Yew K, Sharma R, Duncan R, Saleem M, et al. LPS and PAN-induced podocyte injury in an in vitro model of minimal change disease: changes in TLR profile. J Cell Commun Signal. 2013;7:49-60 pubmed publisher

product information

Catalog Number :

04-235

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-NFĸB Antibody, p65, clone E379, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P19838

Host Name :

Rabbit

Antigen :

NFĸB

Clone :

E379

Conjugate :

Culture Supernatant

Isotype :

IgG

Product Description :

Anti-NFĸB Antibody, p65, clone E379, rabbit monoclonal

Cross Reactivity :

Human

Background :

The transcription factor NFĸB (Nuclear Factor kappa B) is involved in the expression and regulation of a number of important cellular and physiological processes such as growth, development, apoptosis, immune and inflammatory response. NFĸB represents a group of 5 structurally related and evolutionarily conserved proteins in mammals that include Rel (cRel), RelA (p65), RelB, NFκB1 (p50 and its precursor p105), and NFκB2 (p52 and its precursor p100). NFκB/Rel proteins exist as homo- or heterodimers to form transcriptionally competent or repressive complexes, though the p50/p65 heterodimer of NFκB appears to be the most abundant in cells. A critical component in NFκB regulation is the IκB Kinase (IKK) complex. NFκB transcription factors exist in their inactive form and are retained in the cytoplasm by the bound inhibitory IκB proteins. Upon stimulation by multiple inducers including viruses or cytokines, IκB is rapidly phosphorylated and degraded, resulting in the release of the NFκB complex. The p105 subunit is cleaved into its active p50 form. This cleavage exposes the NLS sequence on the p50 subunit. The p50/p65 heteroduplex then translocates to the nucleus where it activates gene transcription. The wide variety of genes regulated by NFκB includes those encoding cytokines, chemokines, adhesion molecules, acute phase proteins and inducible effector enzymes.

ALT Names :

Nuclear Factor kappa B

Immunogen :

KLH conjugated to NFκB p65 linear peptide

Specificity :

This antibody recognizes NFκB p65.

Package Size :

100 μL

Uses :

Flow Cytometry;Immunocytochemistry;Immunohistochemistry;Immunohistochemistry (Paraffin);Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20ºC from date of receipt. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage