NFĸB p50 Antibody, clone E381, rabbit monoclonal | EMD Millipore 04-234 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

NFĸB p50 Antibody, clone E381, rabbit monoclonal

catalog :

04-234

quantity :

100 μL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

E381

The same clone is also sold as:

Abcam: ab32360, ab195854

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1,000	Panguluri S, Tur J, Fukumoto J, Deng W, Sneed K, Kolliputi N, et al. Hyperoxia-induced hypertrophy and ion channel remodeling in left ventricle. Am J Physiol Heart Circ Physiol. 2013;304:H1651-61 pubmed publisher
	Vaque J, Gomez Lopez G, Monsálvez V, Varela I, Martinez N, Perez C, et al. PLCG1 mutations in cutaneous T-cell lymphomas. Blood. 2014;123:2034-43 pubmed publisher

product information

Catalog Number :

04-234

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-NFĸB p50 Antibody, clone E381, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P19838

Host Name :

Rabbit

Antigen :

NFĸB p50

Clone :

E381

Conjugate :

Culture Supernatant

Isotype :

IgG

Product Description :

Anti-NFĸB p50 Antibody, clone E381, rabbit monoclonal

Cross Reactivity :

Human;Mouse;Rat

Background :

The transcription factor NFĸB (Nuclear Factor kappa B) is involved in the expression and regulation of a number of important cellular and physiological processes such as growth, development, apoptosis, immune and inflammatory response. NFĸB family represents a group of 5 structurally related and evolutionarily conserved proteins in mammals that include Rel (cRel), RelA (p65), RelB, NFĸB1 (p50 and its precursor p105), and NFĸB2 (p52 and its precursor p100). NFĸB/Rel proteins exist as homo- or heterodimers to form transcriptionally competent or repressive complexes, though the p50/p65 heterodimer of NFĸB appears to be the most abundant in cells. A critical component in NFĸB regulation is the IĸB Kinase (IKK) complex. NFĸB transcription factors exist in their inactive form and are retained in the cytoplasm by the bound inhibitory IĸB proteins. Upon stimulation, IĸB is rapidly phosphorylated and degraded, resulting in the release of the NFĸB complex. The p105 subunit is cleaved into its active p50 form. This cleavage exposes the NLS sequence on the p50 subunit. p50/p65 then translocates to the nucleus where it activates gene transcription. The wide variety of genes regulated by NFĸB includes those encoding cytokines, chemokines, adhesion molecules, acute phase proteins and inducible effector enzymes.

ALT Names :

Nuclear Factor kappa B

Immunogen :

KLH conjugated to NFB p105/50 linear peptide.

Specificity :

This antibody recognizes NFκB p50 and its p105 uncleaved precursor.

Package Size :

100 μL

Uses :

Flow Cytometry;Immunocytochemistry;Immunohistochemistry;Immunohistochemistry (Paraffin);Western Blotting

Storage :

Stable for 1 year at -20ºC from date of receipt. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.