CREB Antibody, clone E306, rabbit monoclonal | EMD Millipore 04-218 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

CREB Antibody, clone E306, rabbit monoclonal

catalog :

04-218

quantity :

100 μL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

E306

The same clone is also sold as:

Abcam: ab32515

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

citations: 2

Reference
Basavarajappa B, Nagre N, Xie S, Subbanna S. Elevation of endogenous anandamide impairs LTP, learning, and memory through CB1 receptor signaling in mice. Hippocampus. 2014;24:808-18 pubmed publisher
D Amico A, Scuderi S, Leggio G, Castorina A, Drago F, D Agata V. Increased hippocampal CREB phosphorylation in dopamine D3 receptor knockout mice following passive avoidance conditioning. Neurochem Res. 2013;38:2516-23 pubmed publisher

product information

Catalog Number :

04-218

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-CREB Antibody, clone E306, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P16220

Host Name :

Rabbit

Antigen :

CREB

Clone :

E306

Conjugate :

Culture Supernatant

Isotype :

IgG

Product Description :

Anti-CREB Antibody, clone E306, rabbit monoclonal

Cross Reactivity :

Human;Mouse;Rat

Background :

CREB, cyclic AMP (cAMP) response element-binding protein, is a ubiquitously expressed basic leucine zipper transcription factor that functions in glucose homeostasis and stimulates the expression of numerous genes in response to growth factors, hormones, neurotransmitters, ion fluxes, stress signals, and has been implicated in memory because if its apparent role in synaptic plasticity. CREB functions either by itself or with related family members, which include CREB, CREM, and ATF-1, by forming homo- and heterodimers through their basic leucine zipper (bZIP) domains. The CREB signaling pathway is activated by extracellular ligands that bind to cell surface receptors and relay their message through various intracellular second messengers resulting ultimately in the activation of nuclear CREB through phosphorylation of the transactivation domain at serine 1332,4. The classical model of CREB activation is through PKA (cAMP-dependent protein kinase). In the non-active state, PKA resides in the cytoplasm as an inactive heterotetramer of paired regulatory and catalytic subunits. Stimulation of cAMP causes the releases of the catalytic subunits of PKA that passively diffuse into the nucleus and initiates gene expression by phosphorylating CREB at its transactivation domain on serine 1331,2,3,4. CREB mediates the activation of cAMP-responsive genes by binding as a dimer to a conserved cAMP-responsive element (CRE), 5’-TGACGTCA-3’, located within the promoter region of target genes. Target gene activation is terminated by the serine/threonine phosphatases PP-1-mediated dephospho-rylation of CREB.

ALT Names :

cAMP responsive element binding protein ;active transcription factor CREB;transactivator protein

Immunogen :

KLH conjugated to CREB linear peptide.

Specificity :

This antibody recognizes CREB.

Package Size :

100 μL

Uses :

Flow Cytometry;Immunocytochemistry;Immunofluorescence;Immunohistochemistry (Paraffin);Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.