phospho-c-Jun (Ser63) Antibody,clone Y172, rabbit monoclonal | EMD Millipore 04-212 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-c-Jun (Ser63) Antibody,clone Y172, rabbit monoclonal

catalog :

04-212

quantity :

100 μL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

Y172

The same clone is also sold as:

Abcam: ab32385, ab227533

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; human; tbl s3	Cheng H, Liang Y, Kuo Y, Chuu C, Lin C, Lee M, et al. Identification of thioridazine, an antipsychotic drug, as an antiglioblastoma and anticancer stem cell agent using public gene expression data. Cell Death Dis. 2015;6:e1753 pubmed publisher
	Chellappa K, Jankova L, Schnabl J, Pan S, Brelivet Y, Fung C, et al. Src tyrosine kinase phosphorylation of nuclear receptor HNF4? correlates with isoform-specific loss of HNF4? in human colon cancer. Proc Natl Acad Sci U S A. 2012;109:2302-7 pubmed publisher

product information

Catalog Number :

04-212

Subcategory :

Cell Signaling

Product Name :

Anti-phospho-c-Jun (Ser63) Antibody,clone Y172, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P05412

Host Name :

Rabbit

Antigen :

phospho-c-Jun (Ser63)

Clone :

Y172

Conjugate :

Culture Supernatant

Isotype :

IgG

Product Description :

Anti-phospho-c-Jun (Ser63) Antibody,clone Y172, rabbit monoclonal

Cross Reactivity :

Human;Mouse;Rat

Background :

Jun is a component of the transcription factor AP-1, and can constitute AP-1 activity either as a homodimer or as a heterodimer with Fos. By contrast with Fos, Jun is expressed constitutively, and can be activated by phosphorylation of serines 63 and/or 73. These sites are targets for the SAPK1/JNK family of protein kinases, and their phosphorylation states can be monitored using antibodies specific for the phospho-epitopes. In addition, Jun is phosphorylated by GSK3 on sites which inhibit Jun activity. Since GSK3 is inactivated by Akt, the Akt pathway results in Jun activation.

ALT Names :

Activator protein 1;Jun activation domain binding protein;Proto-oncogene c-jun;V-jun avian sarcoma virus 17 oncogene homolog ;enhancer-binding protein AP1;jun oncogene;v-jun sarcoma virus 17 oncogene homolog

Immunogen :

Synthetic peptide directed against phosphoylated Ser63 of human c-Jun and surrounding amino acids.

Specificity :

This antibody recognizes phosphorylated c-Jun at and around Ser63.

Package Size :

100 μL

Uses :

Immunocytochemistry;Immunohistochemistry;Immunohistochemistry (Paraffin);Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.