Chk1 (NT) Antibody, clone E250, rabbit monoclonal | EMD Millipore 04-207 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Chk1 (NT) Antibody, clone E250, rabbit monoclonal

catalog :

04-207

quantity :

100 μL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

E250

reactivity :

human, mouse

application :

western blot, immunocytochemistry

citations: 2

Reference
Ramirez Y, Mladek A, Phillips R, Gynther M, Rautio J, Ross A, et al. Evaluation of novel imidazotetrazine analogues designed to overcome temozolomide resistance and glioblastoma regrowth. Mol Cancer Ther. 2015;14:111-9 pubmed publisher
Sáez Ayala M, Fernández Pérez M, Montenegro M, Sánchez del Campo L, Chazarra S, Piñero Madrona A, et al. Melanoma coordinates general and cell-specific mechanisms to promote methotrexate resistance. Exp Cell Res. 2012;318:1146-59 pubmed publisher

product information

Catalog Number :

04-207

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Chk1 (NT) Antibody, clone E250, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

O14757

Host Name :

Rabbit

Antigen :

Chk1 (NT)

Clone :

E250

Conjugate :

Culture Supernatant

Isotype :

IgG

Product Description :

Anti-Chk1 (NT) Antibody, clone E250, rabbit monoclonal

Cross Reactivity :

Human;Rat

Background :

DNA damage activates ATM, which phosphorylates and activates CHK1 and CHK2/Cds1. The principal substrates of CHK1 and CHK2 are cdc25C (inhibited by phosphorylation), p53 (stabilized by phosphorylation), and BRCA1 (scatters through the nucleus following phosphorylation). Activation of p53 results in cell cycle arrest, or induction of apoptosis. Cdc25C inhibition prevents dephosphorylation of the inhibitory phosphotyrosine residue on cdc2/cdk1, thus preventing the G2/M transition. BRCA1 dispersion through the nucleus is associated with DNA repair.

ALT Names :

CHK1 (checkpoint, S.pombe) homolog;CHK1 checkpoint homolog;CHK1 checkpoint homolog (S. pombe);Checkpoint, S. pombe, homolog of, 1

Immunogen :

Synthetic peptide directed against the N-terminus of human Chk1.

Specificity :

This antibody recognizes Chk1 at the N-Terminus.

Package Size :

100 μL

Uses :

Western Blotting;Immunocytochemistry

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.