ATM Antibody, clone Y170, rabbit monoclonal | EMD Millipore 04-200 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

ATM Antibody, clone Y170, rabbit monoclonal

catalog :

04-200

quantity :

100 μL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

Y170

The same clone is also sold as:

Abcam: ab216617, ab32420

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, proximity ligation assay

citations: 4

Published Application/Species/Sample/Dilution	Reference
proximity ligation assay; human; loading ...; fig 3c	Hu Y, Li W, Gao T, Cui Y, Jin Y, Li P, et al. The Severe Acute Respiratory Syndrome Coronavirus Nucleocapsid Inhibits Type I Interferon Production by Interfering with TRIM25-Mediated RIG-I Ubiquitination. J Virol. 2017;91: pubmed publisher
immunohistochemistry; human; loading ...; fig 7a western blot; human; loading ...; fig 3a,3c,3e	Weber A, Drobnitzky N, Devery A, Bokobza S, Adams R, Maughan T, et al. Phenotypic consequences of somatic mutations in the ataxia-telangiectasia mutated gene in non-small cell lung cancer. Oncotarget. 2016;7:60807-60822 pubmed publisher
western blot; human; fig 4b	Staquicini F, Qian M, Salameh A, Dobroff A, Edwards J, Cimino D, et al. Receptor tyrosine kinase EphA5 is a functional molecular target in human lung cancer. J Biol Chem. 2015;290:7345-59 pubmed publisher
	Shkreta L, Michelle L, Toutant J, Tremblay M, Chabot B. The DNA damage response pathway regulates the alternative splicing of the apoptotic mediator Bcl-x. J Biol Chem. 2011;286:331-40 pubmed publisher

product information

Catalog Number :

04-200

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-ATM Antibody, clone Y170, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q13315

Host Name :

Rabbit

Antigen :

ATM

Clone :

Y170

Conjugate :

Culture Supernatant

Isotype :

IgG

Product Description :

Anti-ATM Antibody, clone Y170, rabbit monoclonal

Cross Reactivity :

Human

Background :

ATM (Ataxia Telangiectasia Mutated kinase) and ATR (Ataxia Telangiectasia and Rad3-related kinase) are related kinases that regulate cell cycle checkpoints and DNA repair. ATM is activated in response to DNA damage and serves to arrest further cell division before the damage can be repaired. Mutation in the ATM gene results in the autosomal recessive disease ataxia telangiectasia (AT). The identified substrates for ATM are p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1 and Chk1. ATM activates p53, increasing p21/Cip1/Waf1 levels, thus blocking activation of Cdk2. That results in Rb hypophosphorylation and blockage of the G1/S transition. Separately, ATM also phosphorylates and activates Chk1, which phosphorylates Cdc25C. This inactivates Cdc25C and prevents it from dephosphorylating the inhibitory phosphotyrosine residue on cdc2/Cdk1, thus preventing the G2/M transition. The complex phenotype of cells derived from patients with AT suggests that ATM has additional cellular substrates. In unirradiated cells, ATM is present as an inactive homodimer or multimer. Double-stranded breaks in DNA caused by ionizing radiation cause rapid ATM kinase activation through dissociation of this complex and ATM autophosphorylation at Ser1981.

ALT Names :

A-T, mutated;AT mutated;TEL1, telomere maintenance 1, homolog;ataxia telangiectasia mutated;ataxia telangiectasia mutated (includes complementation groups A, C and D);ataxia telangiectasia mutated protein ;human phosphatidylinositol 3-kinase homolog;serine-protein kinase ATM

Immunogen :

Synthetic peptide corresponding to the FAT domain encompassing the area of Ser1981 of human ATM.

Specificity :

This antibody recognizes ATM irrespective of phosphorylation on Ser1981.

Package Size :

100 μL

Uses :

Immunocytochemistry;Immunofluorescence;Immunohistochemistry;Immunohistochemistry (Paraffin);Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.