RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8 | EMD Millipore 04-1572 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8

catalog :

04-1572

quantity :

100 μg

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

3E8

reactivity :

human, mouse, brewer's yeast

application :

western blot, ELISA, chromatin immunoprecipitation, ChIP-Seq

citations: 9

Published Application/Species/Sample/Dilution	Reference
western blot; brewer's yeast; 1:1000; loading ...	Fischl H, Howe F, Furger A, Mellor J. Paf1 Has Distinct Roles in Transcription Elongation and Differential Transcript Fate. Mol Cell. 2017;65:685-698.e8 pubmed publisher
western blot; brewer's yeast; loading ...; fig s1a	McCann T, Guo Y, McDonald W, Tansey W. Antagonistic roles for the ubiquitin ligase Asr1 and the ubiquitin-specific protease Ubp3 in subtelomeric gene silencing. Proc Natl Acad Sci U S A. 2016;113:1309-14 pubmed publisher
chromatin immunoprecipitation; human; fig 2	Jimeno GonzÃ¡lez S, Ceballos ChÃ¡vez M, Reyes J. A positioned +1 nucleosome enhances promoter-proximal pausing. Nucleic Acids Res. 2015;43:3068-78 pubmed publisher
chromatin immunoprecipitation; mouse; loading ...; fig 1	Takahashi J, Kumar V, Nakashe P, Koike N, Huang H, Green C, et al. ChIP-seq and RNA-seq methods to study circadian control of transcription in mammals. Methods Enzymol. 2015;551:285-321 pubmed publisher
western blot; human; fig 2	Liang K, Gao X, Gilmore J, Florens L, Washburn M, Smith E, et al. Characterization of human cyclin-dependent kinase 12 (CDK12) and CDK13 complexes in C-terminal domain phosphorylation, gene transcription, and RNA processing. Mol Cell Biol. 2015;35:928-38 pubmed publisher
ChIP-Seq; human	Menafra R, Brinkman A, Matarese F, Franci G, Bartels S, Nguyen L, et al. Genome-wide binding of MBD2 reveals strong preference for highly methylated loci. PLoS ONE. 2014;9:e99603 pubmed publisher
chromatin immunoprecipitation; mouse	Koike N, Yoo S, Huang H, Kumar V, Lee C, Kim T, et al. Transcriptional architecture and chromatin landscape of the core circadian clock in mammals. Science. 2012;338:349-54 pubmed publisher
	Olson C, Liang Y, Leggett A, Park W, Li L, Mills C, et al. Development of a Selective CDK7 Covalent Inhibitor Reveals Predominant Cell-Cycle Phenotype. Cell Chem Biol. 2019;26:792-803.e10 pubmed publisher
	Wang Y, Ratna P, Shivashankar G. Superresolution imaging of nanoscale chromosome contacts. Sci Rep. 2017;7:42422 pubmed publisher

product information

Catalog Number :

04-1572

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P24928

Host Name :

Rat

Antigen :

RNA polymerase II subunit B1 (phospho-CTD Ser-5)

Clone :

3E8

Conjugate :

Purified

Isotype :

IgG2aκ

Product Description :

Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8

Cross Reactivity :

Mouse;Human

Background :

RNA polymerase II subunit B1 (RPB1) is the largest subunit of the RNA polymerase II complex. As a holoenzyme RNA polymerase II catalyzes transcription of eukaryotic DNA into RNA using the four ribonucleoside triphosphates as substrates. The RBP1 subunit, in combination with other polymerase subunits, forms a large central cleft that maintains contact between the active site of the enzyme, the DNA template, and the nascent RNA transcript. This subunit also contains a carboxy terminal domain (CTD) consisting of tandem heptapeptide repeats. Phosphorylation activates the RNA polymerase II beta subunit, allowing it to serve as an assembly platform for additional subunits that modulate initiation, elongation, termination and mRNA processing. In actively transcribing RNA polymerase ‘Ser-2’ and ‘Ser-5’ of the heptapeptide repeat are phosphorylated. Ser-7 is phosphorylated before initiation of transcription at promoter regions.

ALT Names :

DNA-directed RNA polymerase II A;DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit;DNA-directed RNA polymerase II subunit A;DNA-directed RNA polymerase III largest subunit;RNA polymerase II subunit B1;RNA-directed RNA polymerase II subunit RPB1;polymerase (RNA) II (DNA directed) polypeptide A (220kD);polymerase (RNA) II (DNA directed) polypeptide A, 220kDa

Immunogen :

Ovalbumin-conjugated linear peptide corrresponding to human RNA polymerase subunit B1 CTD phosphorylated at Ser5.

Specificity :

This antibody recognizes RNA polymerase II subunit B1 at the CTD when phosphorylated at Ser5.

Package Size :

100 μg

Uses :

Chromatin Immunoprecipitation (ChIP);Western Blotting;ELISA

Storage :

Stable for 1 year at 2-8°C from date of receipt