RNA polymerase II subunit B1 (phospho CTD Ser-2) Antibody, clone 3E10 | EMD Millipore 04-1571-I product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

RNA polymerase II subunit B1 (phospho CTD Ser-2) Antibody, clone 3E10

catalog :

04-1571-I

quantity :

100 µL

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

3E10

The same clone is also sold as:

Invitrogen: 14-9802-82

reactivity :

human, mouse

application :

western blot, ELISA, chromatin immunoprecipitation

citations: 15

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 2d	Yu D, Cattoglio C, Xue Y, Zhou Q. A complex between DYRK1A and DCAF7 phosphorylates the C-terminal domain of RNA polymerase II to promote myogenesis. Nucleic Acids Res. 2019;47:4462-4475 pubmed publisher
western blot; human; 1:1000; loading ...; fig e3b	Sharifnia T, Wawer M, Chen T, Huang Q, Weir B, Sizemore A, et al. Small-molecule targeting of brachyury transcription factor addiction in chordoma. Nat Med. 2019;25:292-300 pubmed publisher
western blot; human; fig 2	Liang K, Gao X, Gilmore J, Florens L, Washburn M, Smith E, et al. Characterization of human cyclin-dependent kinase 12 (CDK12) and CDK13 complexes in C-terminal domain phosphorylation, gene transcription, and RNA processing. Mol Cell Biol. 2015;35:928-38 pubmed publisher
	Fischl H, Howe F, Furger A, Mellor J. Paf1 Has Distinct Roles in Transcription Elongation and Differential Transcript Fate. Mol Cell. 2017;65:685-698.e8 pubmed publisher
	Rao M, Matsumoto Y, Richardson M, Panneerdoss S, Bhardwaj A, Ward J, et al. Hormone-induced and DNA demethylation-induced relief of a tissue-specific and developmentally regulated block in transcriptional elongation. J Biol Chem. 2014;289:35087-101 pubmed publisher
	Pradeepa M, Grimes G, Taylor G, Sutherland H, Bickmore W. Psip1/Ledgf p75 restrains Hox gene expression by recruiting both trithorax and polycomb group proteins. Nucleic Acids Res. 2014;42:9021-32 pubmed publisher
	Menafra R, Brinkman A, Matarese F, Franci G, Bartels S, Nguyen L, et al. Genome-wide binding of MBD2 reveals strong preference for highly methylated loci. PLoS ONE. 2014;9:e99603 pubmed publisher
	Hsin J, Xiang K, Manley J. Function and control of RNA polymerase II C-terminal domain phosphorylation in vertebrate transcription and RNA processing. Mol Cell Biol. 2014;34:2488-98 pubmed publisher
	Hérissant L, Moehle E, Bertaccini D, Van Dorsselaer A, Schaeffer Reiss C, Guthrie C, et al. H2B ubiquitylation modulates spliceosome assembly and function in budding yeast. Biol Cell. 2014;106:126-38 pubmed publisher
	Lenstra T, Tudek A, Clauder S, Xu Z, Pachis S, van Leenen D, et al. The role of Ctk1 kinase in termination of small non-coding RNAs. PLoS ONE. 2013;8:e80495 pubmed publisher
	Mukundan B, Ansari A. Srb5/Med18-mediated termination of transcription is dependent on gene looping. J Biol Chem. 2013;288:11384-94 pubmed publisher
	Devaiah B, Singer D. Cross-talk among RNA polymerase II kinases modulates C-terminal domain phosphorylation. J Biol Chem. 2012;287:38755-66 pubmed publisher
	Fuda N, Buckley M, Wei W, Core L, Waters C, Reinberg D, et al. Fcp1 dephosphorylation of the RNA polymerase II C-terminal domain is required for efficient transcription of heat shock genes. Mol Cell Biol. 2012;32:3428-37 pubmed publisher
	Guth A, Hafeman S, Elmslie R, Dow S. Liposomal clodronate treatment for tumour macrophage depletion in dogs with soft-tissue sarcoma. Vet Comp Oncol. 2013;11:296-305 pubmed publisher
	Devaiah B, Lewis B, Cherman N, Hewitt M, Albrecht B, Robey P, et al. BRD4 is an atypical kinase that phosphorylates serine2 of the RNA polymerase II carboxy-terminal domain. Proc Natl Acad Sci U S A. 2012;109:6927-32 pubmed publisher

product information

Catalog Number :

04-1571-I

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-RNA polymerase II subunit B1 (phospho CTD Ser-2) Antibody, clone 3E10

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P24928

Host Name :

Rat

Antigen :

RNA polymerase II subunit B1 (phospho CTD Ser-2)

Clone :

3E10

Conjugate :

Culture Supernatant

Isotype :

IgG1κ

Product Description :

Anti-RNA polymerase II subunit B1 (phospho CTD Ser-2) Antibody, clone 3E10

Cross Reactivity :

Mouse;Human

Background :

RPB1 (RNA polymerase II subunit B1) is the catalytic component of RNA polymerase II which synthesizes mRNA and non-coding RNAs. During transcription, elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of RPB1, which acts as an assembly platform for factors regulating transcription initiation, elongation, termination and mRNA processing. Phosphorylation occurs mainly at residues 'Ser-2' and 'Ser-5' of the tandem 7 residue repeats in the C-terminal domain (CTD), which activates Pol II. This phosphorylation also can occur at 'Ser-7' of the heptapepdtide repeat. This antibody recognizes the 'Ser-2' CTD residue of RPB1.

ALT Names :

DNA-directed RNA polymerase II subunit RPB1;RNA polymerase II subunit B1;DNA-directed RNA polymerase II subunit A;DNA-directed RNA polymerase III largest subunit;RNA-directed RNA polymerase II subunit RPB1

Immunogen :

Ovalbumin-conjugated linear peptide corresponding to human RNA Polymerase II subunit B1 phosphorylated at Ser2 of the C-terminal domain (CTD).

Specificity :

This antibody recognizes RNA Polymerase II subunit B1 phosphorylated at Ser2 of the C-terminal domain (CTD).

Package Size :

100 µL

Uses :

Chromatin Immunoprecipitation (ChIP);Western Blotting;ELISA

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.