RNA polymerase II subunit B1 (phospho-CTD Ser-7) Antibody, clone 4E12 | EMD Millipore 04-1570-I product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

RNA polymerase II subunit B1 (phospho-CTD Ser-7) Antibody, clone 4E12

catalog :

04-1570-I

quantity :

100 µL

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

4E12

reactivity :

human, mouse

application :

western blot, ELISA, chromatin immunoprecipitation

citations: 9

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; loading ...; fig e3b	Sharifnia T, Wawer M, Chen T, Huang Q, Weir B, Sizemore A, et al. Small-molecule targeting of brachyury transcription factor addiction in chordoma. Nat Med. 2019;25:292-300 pubmed publisher
western blot; human; fig 2	Liang K, Gao X, Gilmore J, Florens L, Washburn M, Smith E, et al. Characterization of human cyclin-dependent kinase 12 (CDK12) and CDK13 complexes in C-terminal domain phosphorylation, gene transcription, and RNA processing. Mol Cell Biol. 2015;35:928-38 pubmed publisher
	Olson C, Liang Y, Leggett A, Park W, Li L, Mills C, et al. Development of a Selective CDK7 Covalent Inhibitor Reveals Predominant Cell-Cycle Phenotype. Cell Chem Biol. 2019;26:792-803.e10 pubmed publisher
	Fischl H, Howe F, Furger A, Mellor J. Paf1 Has Distinct Roles in Transcription Elongation and Differential Transcript Fate. Mol Cell. 2017;65:685-698.e8 pubmed publisher
	Hsin J, Xiang K, Manley J. Function and control of RNA polymerase II C-terminal domain phosphorylation in vertebrate transcription and RNA processing. Mol Cell Biol. 2014;34:2488-98 pubmed publisher
	Lenstra T, Tudek A, Clauder S, Xu Z, Pachis S, van Leenen D, et al. The role of Ctk1 kinase in termination of small non-coding RNAs. PLoS ONE. 2013;8:e80495 pubmed publisher
	Elfrink H, Zwart R, Baas F, Scheper W. Inhibition of endoplasmic reticulum associated degradation reduces endoplasmic reticulum stress and alters lysosomal morphology and distribution. Mol Cells. 2013;35:291-7 pubmed publisher
	Xiang K, Manley J, Tong L. An unexpected binding mode for a Pol II CTD peptide phosphorylated at Ser7 in the active site of the CTD phosphatase Ssu72. Genes Dev. 2012;26:2265-70 pubmed publisher
	St Amour C, Sansó M, Bösken C, Lee K, Larochelle S, Zhang C, et al. Separate domains of fission yeast Cdk9 (P-TEFb) are required for capping enzyme recruitment and primed (Ser7-phosphorylated) Rpb1 carboxyl-terminal domain substrate recognition. Mol Cell Biol. 2012;32:2372-83 pubmed publisher

product information

Catalog Number :

04-1570-I

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7) Antibody, clone 4E12

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P24928

Host Name :

Rat

Antigen :

RNA polymerase II subunit B1 (phospho-CTD Ser-7)

Clone :

4E12

Conjugate :

Culture Supernatant

Isotype :

IgG1κ

Product Description :

Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7) Antibody, clone 4E12

Cross Reactivity :

Mouse;Human

Background :

RPB1 (RNA polymerase II subunit B1) is the catalytic component of RNA polymerase II which synthesizes mRNA and non-coding RNAs. During transcription, elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of RPB1, which acts as an assembly platform for factors regulating transcription initiation, elongation, termination and mRNA processing. Phosphorylation occurs mainly at residues 'Ser-2' and 'Ser-5' of the tandem 7 residue repeats in the C-terminal domain (CTD), which activates Pol II. This phosphorylation also can occur at 'Ser-7' of the heptapepdtide repeat. This antibody recognizes the 'Ser-7' CTD residue of RPB1.

ALT Names :

DNA-directed RNA polymerase II subunit RPB1;RNA polymerase II subunit B1;DNA-directed RNA polymerase II subunit A;DNA-directed RNA polymerase III largest subunit;RNA-directed RNA polymerase II subunit RPB1

Immunogen :

Ovalbumin-conjugated linear peptide corresponding to human RNA polymerase II subunit B1 phosphorylated at Ser7 of the C-terminal domain (CTD).

Specificity :

This antibody recognizes RNA polymerase II subunit B1 phosphorylated at Ser7 of the C-terminal domain (CTD).

Package Size :

100 µL

Uses :

Chromatin Immunoprecipitation (ChIP);Western Blotting;ELISA

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.