SIRT1 Antibody, clone 10E4 | EMD Millipore 04-1557 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

SIRT1 Antibody, clone 10E4

catalog :

04-1557

quantity :

200 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

10-E-4

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section

citations: 6

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; fig 5 western blot; human; 1:1000; fig 4	Sarma P, Bag I, Ramaiah M, Kamal A, Bhadra U, Pal Bhadra M. Bisindole-PBD regulates breast cancer cell proliferation via SIRT-p53 axis. Cancer Biol Ther. 2015;16:1486-501 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100; fig 7 western blot; human; 1:1000; fig 6	Sedic M, Skibinski A, Brown N, Gallardo M, Mulligan P, Martinez P, et al. Haploinsufficiency for BRCA1 leads to cell-type-specific genomic instability and premature senescence. Nat Commun. 2015;6:7505 pubmed publisher
western blot; rat; 1:500	Ortiz F, AcuÃ±a Castroviejo D, Doerrier C, Dayoub J, LÃ³pez L, Venegas C, et al. Melatonin blunts the mitochondrial/NLRP3 connection and protects against radiation-induced oral mucositis. J Pineal Res. 2015;58:34-49 pubmed publisher
western blot; mouse western blot; human	Singh B, Sinha R, Zhou J, Xie S, You S, Gauthier K, et al. FoxO1 deacetylation regulates thyroid hormone-induced transcription of key hepatic gluconeogenic genes. J Biol Chem. 2013;288:30365-72 pubmed publisher
	O Callaghan P, Noborn F, Sehlin D, Li J, Lannfelt L, Lindahl U, et al. Apolipoprotein E increases cell association of amyloid-? 40 through heparan sulfate and LRP1 dependent pathways. Amyloid. 2014;21:76-87 pubmed publisher
	Cheng J, Whitelock J, Poole Warren L. Syndecan-4 is associated with beta-cells in the pancreas and the MIN6 beta-cell line. Histochem Cell Biol. 2012;138:933-44 pubmed publisher

product information

Catalog Number :

04-1557

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-SIRT1 Antibody, clone 10E4

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q96EB6

Host Name :

Mouse

Antigen :

SIRT1

Clone :

10-E-4

Conjugate :

Purified

Product Description :

Anti-SIRT1 Antibody, clone 10E4

Cross Reactivity :

Human;Mouse

Background :

Sir2 is short for Silent mating type Information Regulation-2. Sir2 (whose homolog in mammals is known as SIRT1, SIR2L1 or Sir2α) is the namesake of a family of closely related enzymes, the sirtuins. Members of this family have been found in nearly all organisms studied. Sirtuins are hypothesized to play a key role in an organism's response to stresses (such as heat or starvation) and to be responsible for the lifespan-extending effects of calorie restriction. Sirtuins act by removing acetyl groups from proteins in the presence of NAD+; they are thus classified as "NAD+-dependent deacetylases". In mammals, SIRT1 (the mammalian homolog of Sir2) has been shown to deacetylate and thereby deactivate the p53 protein.

ALT Names :

SIR2-like protein 1;sir2-like 1;sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae);sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1;sirtuin 1;sirtuin type 1

Immunogen :

His-tagged recombinant protein corresponding to human SIRT1.

Specificity :

This antibody recognizes SIRT1.

Package Size :

200 µL

Uses :

Western Blotting;Immunoprecipitation;Immunocytochemistry;Immunohistochemistry;Chromatin Immunoprecipitation (ChIP)

Storage :

Stable for 1 year at 2-8°C from date of receipt