Mdmx Antibody, clone 8C6 | EMD Millipore 04-1555 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Mdmx Antibody, clone 8C6

catalog :

04-1555

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8C6

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation

citations: 8

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:300; loading ...; fig 1b	Qi D, Cobrinik D. MDM2 but not MDM4 promotes retinoblastoma cell proliferation through p53-independent regulation of MYCN translation. Oncogene. 2017;36:1760-1769 pubmed publisher
western blot; human; loading ...; fig s8	Chen S, FORRESTER W, Lahav G. Schedule-dependent interaction between anticancer treatments. Science. 2016;351:1204-8 pubmed publisher
immunocytochemistry; human; 1:25; fig 2	Swetzig W, Wang J, Das G. Estrogen receptor alpha (ERÎ±/ESR1) mediates the p53-independent overexpression of MDM4/MDMX and MDM2 in human breast cancer. Oncotarget. 2016;7:16049-69 pubmed publisher
western blot; mouse; 1:1000; fig 1	Dewaele M, Tabaglio T, Willekens K, Bezzi M, Teo S, Low D, et al. Antisense oligonucleotide-mediated MDM4 exon 6 skipping impairs tumor growth. J Clin Invest. 2016;126:68-84 pubmed publisher
western blot; human; 1:1000; loading ...; fig 1b	Deben C, Wouters A, Op de Beeck K, Van den Bossche J, Jacobs J, Zwaenepoel K, et al. The MDM2-inhibitor Nutlin-3 synergizes with cisplatin to induce p53 dependent tumor cell apoptosis in non-small cell lung cancer. Oncotarget. 2015;6:22666-79 pubmed
western blot; human	Ling X, Xu C, Fan C, Zhong K, Li F, Wang X. FL118 induces p53-dependent senescence in colorectal cancer cells by promoting degradation of MdmX. Cancer Res. 2014;74:7487-97 pubmed publisher
western blot; human; 1:1000	Okada N, Lin C, Ribeiro M, Biton A, Lai G, He X, et al. A positive feedback between p53 and miR-34 miRNAs mediates tumor suppression. Genes Dev. 2014;28:438-50 pubmed publisher
	Navarro F, Lieberman J. miR-34 and p53: New Insights into a Complex Functional Relationship. PLoS ONE. 2015;10:e0132767 pubmed publisher

product information

Catalog Number :

04-1555

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Mdmx Antibody, clone 8C6

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

O15151

Host Name :

Mouse

Antigen :

Mdmx

Clone :

8C6

Conjugate :

Purified

Isotype :

IgG2bκ

Product Description :

Anti-Mdmx Antibody, clone 8C6

Cross Reactivity :

Human

Background :

Mdmx is a Mdm2-related protein that like Mdm2 has been shown to bind to and inhibit p53, although not as well as Mdm2. This interaction with p53 occurs via a binding domain located in the N-terminal region of the protein. Mdmx has been shown to counteract Mdm2-dependent p53 degradation and to stabilize p53 in its inactive form. Although Mdmx has significant structural similarity to Mdm2 E3 ubiquitin ligase, most studies have not been able to ascribe E3 ligase function to Mdmx.

ALT Names :

Double minute 4 protein;MDM4-related protein 1;Mdm2-like p53-binding protein;Mdm4 p53 binding protein homolog (mouse);Mdm4, transformed 3T3 cell double minute 4, p53 binding protein;Mdm4, transformed 3T3 cell double minute 4, p53 binding protein (mouse);Protein Mdmx;double minute 4, human homolog of;p53-binding protein;mouse double minute 4 homolog;mouse double minute 4, human homolog of;p53-binding protein;p53-binding protein 2 p53-binding protein Mdm4

Immunogen :

His-tagged recombinant protein corresponding to human Mdmx.

Specificity :

This antibody recognizes Mdmx.

Package Size :

100 μg

Uses :

Western Blotting;Immunoprecipitation;Immunocytochemistry;Immunohistochemistry;Flow Cytometry;Chromatin Immunoprecipitation (ChIP)

Storage :

Stable for 1 year at 2-8°C from date of receipt