MDM2 Antibody, clone 3G9 | EMD Millipore 04-1530 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

MDM2 Antibody, clone 3G9

catalog :

04-1530

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3G9

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, western blot knockout validation, immunohistochemistry knockout validation

citations: 7

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry knockout validation; mouse; 1:50; fig 1 western blot knockout validation; mouse; 1:500; fig 1	Li X, Cheng K, Liu Z, Yang J, Wang B, Jiang X, et al. The MDM2-p53-pyruvate carboxylase signalling axis couples mitochondrial metabolism to glucose-stimulated insulin secretion in pancreatic β-cells. Nat Commun. 2016;7:11740 pubmed publisher
western blot; mouse; 1:2500; loading ...; fig 10a	Kalita B, Ranjan R, Singh A, Yashavarddhan M, Bajaj S, Gupta M. A Combination of Podophyllotoxin and Rutin Attenuates Radiation Induced Gastrointestinal Injury by Negatively Regulating NF-?B/p53 Signaling in Lethally Irradiated Mice. PLoS ONE. 2016;11:e0168525 pubmed publisher
western blot; human; fig 3	Tonsing Carter E, Bailey B, Saadatzadeh M, Ding J, Wang H, Sinn A, et al. Potentiation of Carboplatin-Mediated DNA Damage by the Mdm2 Modulator Nutlin-3a in a Humanized Orthotopic Breast-to-Lung Metastatic Model. Mol Cancer Ther. 2015;14:2850-63 pubmed publisher
western blot; mouse; 1:500; fig 4b	Pencik J, Schlederer M, Gruber W, Unger C, Walker S, Chalaris A, et al. STAT3 regulated ARF expression suppresses prostate cancer metastasis. Nat Commun. 2015;6:7736 pubmed publisher
immunoprecipitation; human; 2 ugs western blot; human; 1:2000	Heyne K, Förster J, Schüle R, Roemer K. Transcriptional repressor NIR interacts with the p53-inhibiting ubiquitin ligase MDM2. Nucleic Acids Res. 2014;42:3565-79 pubmed publisher
	Deben C, Wouters A, Op de Beeck K, Van den Bossche J, Jacobs J, Zwaenepoel K, et al. The MDM2-inhibitor Nutlin-3 synergizes with cisplatin to induce p53 dependent tumor cell apoptosis in non-small cell lung cancer. Oncotarget. 2015;6:22666-79 pubmed
	Wang Y, Huang J, Castella M, Huntsman D, Taniguchi T. p53 is positively regulated by miR-542-3p. Cancer Res. 2014;74:3218-27 pubmed publisher

product information

Catalog Number :

04-1530

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-MDM2 Antibody, clone 3G9

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q00987

Host Name :

Mouse

Antigen :

MDM2

Clone :

3G9

Conjugate :

Purified

Product Description :

Anti-MDM2 Antibody, clone 3G9

Cross Reactivity :

Human;Mouse

Background :

MDM2 is over-expressed in many tumors. Its principal function is the ubiquitination and degradation of p53 tumor suppressor protein. This monoclonal antibody also recognizes a peptide epitope around Thr-216 of murine MDM2 when Thr-216 is unphosphorylated. SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.

ALT Names :

Double minute 2 protein;Mdm2 p53 binding protein homolog (mouse);Mdm2, transformed 3T3 cell double minute 2, p53 binding protein;Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse);Oncoprotein Mdm2;double minute 2, human homolog of;p53-binding protein;mouse double minute 2 homolog;mouse double minute 2, human homolog of;p53-binding protein;p53-binding protein Mdm2;ubiquitin-protein ligase E3 Mdm2

Immunogen :

His-tagged recombinant protein corresponding to human MDM2.

Specificity :

This antibody recognizes MDM2.

Package Size :

100 μg

Uses :

Western Blotting;Immunoprecipitation;Immunocytochemistry;Immunohistochemistry

Storage :

Stable for 1 year at 2-8°C from date of receipt