methyl-PP2A Antibody, C subunit, clone 2A10 | EMD Millipore 04-1479 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

methyl-PP2A Antibody, C subunit, clone 2A10

catalog :

04-1479

quantity :

150 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2A10

The same clone is also sold as:

BioLegend: 828801
Abcam: ab66597
ImmuQuest: IQ556HRP, IQ556
Santa Cruz Biotechnology: sc-81603
Invitrogen: MA5-18072

reactivity :

human, mouse, rat, chicken

application :

western blot

citations: 6

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; fig 4	Sontag J, Wasek B, Taleski G, Smith J, Arning E, Sontag E, et al. Altered protein phosphatase 2A methylation and Tau phosphorylation in the young and aged brain of methylenetetrahydrofolate reductase (MTHFR) deficient mice. Front Aging Neurosci. 2014;6:214 pubmed publisher
western blot; mouse; 1:1000	Chasseigneaux S, Clamagirand C, Huguet L, Gorisse Hussonnois L, Rose C, Allinquant B. Cytoplasmic SET induces tau hyperphosphorylation through a decrease of methylated phosphatase 2A. BMC Neurosci. 2014;15:82 pubmed publisher
western blot; human	Dou X, Xia Y, Chen J, Qian Y, Li S, Zhang X, et al. Rectification of impaired adipose tissue methylation status and lipolytic response contributes to hepatoprotective effect of betaine in a mouse model of alcoholic liver disease. Br J Pharmacol. 2014;171:4073-86 pubmed publisher
western blot; rat	Longman M, Ranieri A, Avkiran M, Snabaitis A. Regulation of PP2AC carboxylmethylation and cellular localisation by inhibitory class G-protein coupled receptors in cardiomyocytes. PLoS ONE. 2014;9:e86234 pubmed publisher
	Preuss K, Fadle N, Regitz E, Held G, Pfreundschuh M. Inactivation of protein-phosphatase 2A causing hyperphosphorylation of autoantigenic paraprotein targets in MGUS/MM is due to an exchange of its regulatory subunits. Int J Cancer. 2014;135:2046-53 pubmed publisher
	Buss M, Read T, Schniederjan M, Gandhi K, Castellino R. HDM2 promotes WIP1-mediated medulloblastoma growth. Neuro Oncol. 2012;14:440-58 pubmed publisher

product information

Catalog Number :

04-1479

Subcategory :

Cell Signaling

Product Name :

Anti-methyl-PP2A Antibody, C subunit, clone 2A10

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P67775

Host Name :

Mouse

Antigen :

methyl-PP2A

Clone :

2A10

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-methyl-PP2A Antibody, C subunit, clone 2A10

Cross Reactivity :

Human;Mouse;Yeast ( S. cerevisiae );Rat;Pig;Rabbit;Chicken;Drosophila

Background :

Type 2 protein serine/threonine phosphatase (PP2A) is a trimer composed of a catalytic C subunit and two regulatory (A and B) subunits; many isoforms for the A and B subunits have been found. PP2A has a high specific activity against the α subunit of phosphorylase kinase and is insensitive to Inhibitor-2. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and by its high sensitivity to inhibition by okadaic acid. It is also thought to be the principal down-modulator of serine/threonine kinase cascades. PP2B requires Ca2+/calmodulin for activity, and is an activator of NFAT transcription factors. PP2C requires Mg2+, but is insensitive to okadaic acid, microcystin LR, or the calmodulin inhibitor trifluoperazine.

Immunogen :

KLH-conjugated, synthetic peptide (C-βA-RRTPDYFL-Ome) corresponding to amino acids 302-309 of human PP2A catalytic subunit. Clone 2A10.

Specificity :

Recognizes the methylated form of PP2A, catalytic subunit.

Package Size :

150 µg

Uses :

Western Blotting

Storage :

Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.