phospho-Chk2 (Thr68), clone E126, Rabbit Monoclonal | EMD Millipore 04-1471 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-Chk2 (Thr68), clone E126, Rabbit Monoclonal

catalog :

04-1471

quantity :

100 uL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

E126

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

citations: 1

Reference
Lee K, Jeong J, Kim I, Kim K, Ju B. Cyclo(phenylalanine-proline) induces DNA damage in mammalian cells via reactive oxygen species. J Cell Mol Med. 2015;19:2851-64 pubmed publisher

product information

Catalog Number :

04-1471

Category :

Antibodies

Subcategory :

Cell Signaling

Product Name :

Anti-phospho-Chk2 (Thr68), clone E126, Rabbit Monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal

Gene ID :

NP_001005735.1

Host Name :

Clone :

E126

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-phospho-Chk2 (Thr68), clone E126, Rabbit Monoclonal

Cross Reactivity :

Human

Species Summary :

Human

Background :

The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including Chk2, p53R2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. The checkpoint kinase Chk2 is the mammalian homologue of yeast Cds1/Rad53. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53. Chk2 serves as ATM downstream effector to mediate activation of p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene that is mutated in breast and ovarian cancer.

ALT Names :

CHK2 (checkpoint, S.pombe) homolog , CHK2 checkpoint homolog (S. pombe), checkpoint-like protein CHK2 , protein kinase CHK2 , serine/threonine-protein kinase CHK2

Immunogen :

A synthetic phospho-peptide corresponding to residues surrounding Thr68 of human Chk2.

Specificity :

The antibody detects Chk2 phosphorylated on Threonine 68.

Package Size :

100 uL

Uses :

WB, IH(P), IC, IP

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.