ASC Antibody, clone 2EI-7 | EMD Millipore 04-147 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

ASC Antibody, clone 2EI-7

catalog :

04-147

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2EI-7

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, western blot knockout validation

citations: 15

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; mouse; 1:1000; loading ...; fig 1a immunoprecipitation; mouse; loading ...; fig 4j immunocytochemistry; mouse; 1:1000; loading ...; fig 5c	Martin B, Wang C, Zhang C, Kang Z, Gulen M, Zepp J, et al. T cell-intrinsic ASC critically promotes T(H)17-mediated experimental autoimmune encephalomyelitis. Nat Immunol. 2016;17:583-92 pubmed publisher
immunocytochemistry; mouse; 1:100; loading ...; fig 1a	Samir P, Kesavardhana S, Patmore D, Gingras S, Malireddi R, Karki R, et al. DDX3X acts as a live-or-die checkpoint in stressed cells by regulating NLRP3 inflammasome. Nature. 2019;573:590-594 pubmed publisher
western blot; human; loading ...; fig 2j western blot; mouse; loading ...; fig 2a	Zhang C, Jiang M, Zhou H, Liu W, Wang C, Kang Z, et al. TLR-stimulated IRAKM activates caspase-8 inflammasome in microglia and promotes neuroinflammation. J Clin Invest. 2018;128:5399-5412 pubmed publisher
immunohistochemistry; mouse; 1:100; loading ...; fig 7a	de la Roche M, Hamilton C, Mortensen R, Jeyaprakash A, Ghosh S, Anand P. Trafficking of cholesterol to the ER is required for NLRP3 inflammasome activation. J Cell Biol. 2018;217:3560-3576 pubmed publisher
immunoprecipitation; mouse; 1:400; loading ...; fig 8b	Tang T, Lang X, Xu C, Wang X, Gong T, Yang Y, et al. CLICs-dependent chloride efflux is an essential and proximal upstream event for NLRP3 inflammasome activation. Nat Commun. 2017;8:202 pubmed publisher
immunohistochemistry; mouse; loading ...; fig 3b	Zanoni I, Tan Y, Di Gioia M, Broggi A, Ruan J, Shi J, et al. An endogenous caspase-11 ligand elicits interleukin-1 release from living dendritic cells. Science. 2016;352:1232-6 pubmed publisher
western blot; mouse; 1:100; fig 2	Puyang Z, Feng L, Chen H, Liang P, Troy J, Liu X. Retinal Ganglion Cell Loss is Delayed Following Optic Nerve Crush in NLRP3 Knockout Mice. Sci Rep. 2016;6:20998 pubmed publisher
western blot; mouse; 1:500	Chi W, Chen H, Li F, Zhu Y, Yin W, Zhuo Y. HMGB1 promotes the activation of NLRP3 and caspase-8 inflammasomes via NF-ÎºB pathway in acute glaucoma. J Neuroinflammation. 2015;12:137 pubmed publisher
immunocytochemistry; mouse	Man S, Tourlomousis P, Hopkins L, Monie T, Fitzgerald K, Bryant C. Salmonella infection induces recruitment of Caspase-8 to the inflammasome to modulate IL-1? production. J Immunol. 2013;191:5239-46 pubmed publisher
	Lee S, Hirohama M, Noguchi M, Nagata K, Kawaguchi A. Influenza A Virus Infection Triggers Pyroptosis and Apoptosis of Respiratory Epithelial Cells through the Type I Interferon Signaling Pathway in a Mutually Exclusive Manner. J Virol. 2018;92: pubmed publisher
	Zhu W, Cao F, Feng J, Chen H, Wan J, Lu Q, et al. NLRP3 inflammasome activation contributes to long-term behavioral alterations in mice injected with lipopolysaccharide. Neuroscience. 2017;343:77-84 pubmed publisher
	Franklin B, Bossaller L, De Nardo D, Ratter J, Stutz A, Engels G, et al. The adaptor ASC has extracellular and 'prionoid' activities that propagate inflammation. Nat Immunol. 2014;15:727-37 pubmed publisher
	Ippagunta S, Malireddi R, Shaw P, Neale G, Vande Walle L, Green D, et al. The inflammasome adaptor ASC regulates the function of adaptive immune cells by controlling Dock2-mediated Rac activation and actin polymerization. Nat Immunol. 2011;12:1010-6 pubmed publisher
	Keyel P, Heid M, Salter R. Macrophage responses to bacterial toxins: a balance between activation and suppression. Immunol Res. 2011;50:118-23 pubmed publisher
	Barlan A, Griffin T, McGuire K, Wiethoff C. Adenovirus membrane penetration activates the NLRP3 inflammasome. J Virol. 2011;85:146-55 pubmed publisher

product information

Catalog Number :

04-147

Subcategory :

Inflammation & Immunology

Product Name :

Anti-ASC Antibody, clone 2EI-7

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q9ULZ3

Host Name :

Mouse

Antigen :

ASC

Clone :

2EI-7

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-ASC Antibody, clone 2EI-7

Cross Reactivity :

Mouse;Human

Background :

Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK, Bcl10, Apaf-1, ARC, caspase-2 and caspase-9. A novel CARD domain containing protein has been identified in human and mouse and designated ASC and TMS1. Irregular expression of ASC/TMS1 induced apoptosis through activation of caspase-9 and inhibited the survival of human breast cancer cells. Overexpression of ASC/TMS1 induced DNA fragmentation. ASC/TMS1 is expressed in a variety of human and mouse tissues.

ALT Names :

Caspase recruitment domain-containing protein 5;PYD and CARD domain containing;PYD and CARD domain-containing protein;Target of methylation-induced silencing 1;apoptosis-associated speck-like protein containing a CARD;caspase recruitment domain protein 5;target of methylation-induced silencing-1

Immunogen :

Full length recombinant human ASC.

Specificity :

This antibody recognizes ASC.

Package Size :

100 μg

Uses :

Western Blotting;Flow Cytometry

Storage :

Stable for 1 year at 2-8°C from date of receipt