C/EBP β (CT) Antibody, clone E299, rabbit monoclonal | EMD Millipore 04-1153 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

C/EBP β (CT) Antibody, clone E299, rabbit monoclonal

catalog :

04-1153

quantity :

100 µL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

E299

The same clone is also sold as:

Abcam: ab32358

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

citations: 2

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; mouse immunoprecipitation; mouse western blot; mouse	Han Y, Choi Y, Lee S, Jin Y, Cheong H, Lee K. Yin Yang 1 is a multi-functional regulator of adipocyte differentiation in 3T3-L1 cells. Mol Cell Endocrinol. 2015;413:217-27 pubmed publisher
western blot; mouse	Vakili H, Jin Y, Menticoglou S, Cattini P. CCAAT-enhancer-binding protein ? (C/EBP?) and downstream human placental growth hormone genes are targets for dysregulation in pregnancies complicated by maternal obesity. J Biol Chem. 2013;288:22849-61 pubmed publisher

product information

Catalog Number :

04-1153

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-C/EBP β (CT) Antibody, clone E299, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P17676

Host Name :

Rabbit

Antigen :

C/EBP β (CT)

Clone :

E299

Conjugate :

Unpurified

Isotype :

IgG

Product Description :

Anti-C/EBP β (CT) Antibody, clone E299, rabbit monoclonal

Cross Reactivity :

Human;Rat

Background :

The transcription factor CCAAT/Enhancer Binding Protein beta (C/EBPβ, also known as NFIL6) is a highly conserved B-Zip family member. C/EBPβ regulates multiple genes involved in hepatic function, immune function and adipogenesis, and its functions are generally distinct from those of other C/EBP family members (alpha, gamma, delta, epsilon). C/EBPβ is present in cells as three alternate translation products: two transcription activating forms known as LAP1 and LAP2 (~35 and 32 kDa in rodents, ~43 kDa and 40 kDa in humans) and a truncated inhibitory form known as LIP (~20 kDa) which lacks the N-terminal transcription activation domain. C/EBP β is phosphorylated at multiple sites which are determinants of some of its activities (e.g. activation of c-fos, adipogenesis). A recent report shows that it is acetylated at Lys215/216, and others report that it can be sumoylated. C/EBPβ is also acetylated at Lys39 in the transcription activation domain, and mutation of Lys39 impairs its ability to activate transcription of multiple genes, including several involved in adipogenesis.

ALT Names :

C/EBP beta ;CCAAT/enhancer binding protein (C/EBP), beta ;CCAAT/enhancer binding protein beta ;Nuclear factor NF-IL6 ;Transcription factor 5;interleukin 6-dependent DNA-binding protein ;liver-enriched transcriptional activator protein ;nuclear factor of interleukin 6

Immunogen :

Synthetic peptide corresponding to residues in C-terminus of rat C/EBP β.

Specificity :

This antibody can recognize three C/EBP β isoforms (i.e. LAP*, LAP, and LIP).

Package Size :

100 µL

Uses :

Western Blotting;Immunocytochemistry;Immunoprecipitation

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.