alpha-Actin (Smooth Muscle), clone E184, Rabbit Monoclonal | EMD Millipore 04-1094 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

alpha-Actin (Smooth Muscle), clone E184, Rabbit Monoclonal

catalog :

04-1094

quantity :

100 uL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

E184

The same clone is also sold as:

Abcam: ab32575

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

citations: 9

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000	Lee K, Yeo S, Sung C, Kim S. Twist1 is a key regulator of cancer-associated fibroblasts. Cancer Res. 2015;75:73-85 pubmed publisher
immunocytochemistry; human; 1:200	Chang Y, Chang W, Hung K, Yang D, Cheng Y, Liao Y, et al. The generation of induced pluripotent stem cells for macular degeneration as a drug screening platform: identification of curcumin as a protective agent for retinal pigment epithelial cells against oxidative stress. Front Aging Neurosci. 2014;6:191 pubmed publisher
	Ando Y, Matsubara K, Ishikawa J, Fujio M, Shohara R, Hibi H, et al. Stem cell-conditioned medium accelerates distraction osteogenesis through multiple regenerative mechanisms. Bone. 2014;61:82-90 pubmed publisher
	Bastiaans J, van Meurs J, van Holten Neelen C, Nagtzaam N, van Hagen P, Chambers R, et al. Thrombin induces epithelial-mesenchymal transition and collagen production by retinal pigment epithelial cells via autocrine PDGF-receptor signaling. Invest Ophthalmol Vis Sci. 2013;54:8306-14 pubmed publisher
	Traber P, Chou H, Zomer E, Hong F, Klyosov A, Fiel M, et al. Regression of fibrosis and reversal of cirrhosis in rats by galectin inhibitors in thioacetamide-induced liver disease. PLoS ONE. 2013;8:e75361 pubmed publisher
	Lee K, Chan C, Yang Y, Hsieh Y, Huang Y, Lin H. Aliskiren attenuates chronic carbon tetrachloride-induced liver injury in mice. Eur J Clin Invest. 2012;42:1261-71 pubmed publisher
	Ding S, Walton K, Blue R, McNaughton K, Macnaughton K, Magness S, et al. Mucosal healing and fibrosis after acute or chronic inflammation in wild type FVB-N mice and C57BL6 procollagen ?1(I)-promoter-GFP reporter mice. PLoS ONE. 2012;7:e42568 pubmed publisher
	Wang L, Ly C, Ko C, Meyers E, Lawrence D, Bernstein A. uPA binding to PAI-1 induces corneal myofibroblast differentiation on vitronectin. Invest Ophthalmol Vis Sci. 2012;53:4765-75 pubmed publisher
	Liu Y, Lee M, Timani K, He J, Broxmeyer H. Tip110 maintains expression of pluripotent factors in and pluripotency of human embryonic stem cells. Stem Cells Dev. 2012;21:829-33 pubmed publisher

product information

Catalog Number :

04-1094

Category :

Antibodies

Subcategory :

Cell Structure

Product Name :

Anti-alpha-Actin (Smooth Muscle), clone E184, Rabbit Monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal

Gene ID :

P63267

Host Name :

Clone :

E184

Conjugate :

Culture Supernatant

Isotype :

IgG

Product Description :

Anti-alpha-Actin (Smooth Muscle), clone E184, Rabbit Monoclonal

Cross Reactivity :

Human, Mouse, Rat

Species Summary :

Human, mouse, and rat.

Background :

ASM-1 represents an excellent marker for myogenic soft tissue tumors and smooth muscle differentiation. This antibody reacts with many types of smooth muscle cells, such as those present in vascular walls, intestinal muscularis mucosae and propria, myometrium, stroma of various tissues, and is also positive for myoepithelial cells of various glands, notably salivary and mammary gland. Myogenic soft tissues detected include leiomyosarcomas, leiomyomas, and certain stromal cells surrounding infiltrating ductal carcinoma of the breast.

ALT Names :

Smooth muscle gamma-actin , actin, gamma 2, actin, gamma 2, smooth muscle, enteric , actin-like protein , alpha-actin 3, smooth muscle gamma actin

Immunogen :

Synthetic peptide corresponding to N-terminus of human alpha-Actin.

Specificity :

This antibody only detects actin in smooth muscle in immunohistochemistry.

Package Size :

100 uL

Uses :

WB, IH(P), IP, FC

Storage :

Stable for 1 year at -20ºC from date of receipt.Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.