Sir2/SIRT1 Antibody, clone E104, rabbit monoclonal | EMD Millipore 04-1091 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Sir2/SIRT1 Antibody, clone E104, rabbit monoclonal

catalog :

04-1091

quantity :

100 µL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

E104

The same clone is also sold as:

Abcam: ab32441

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section

citations: 2

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; human western blot; human	Zhang B, Chen J, Cheng A, Ko B. Depletion of sirtuin 1 (SIRT1) leads to epigenetic modifications of telomerase (TERT) gene in hepatocellular carcinoma cells. PLoS ONE. 2014;9:e84931 pubmed publisher
	He H, Yu F, Sun C, Luo Y. CBP/p300 and SIRT1 are involved in transcriptional regulation of S-phase specific histone genes. PLoS ONE. 2011;6:e22088 pubmed publisher

product information

Catalog Number :

04-1091

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Sir2/SIRT1 Antibody, clone E104, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q8IXJ6

Host Name :

Rabbit

Antigen :

Sir2/SIRT1

Clone :

E104

Conjugate :

Unpurified

Isotype :

IgG

Product Description :

Anti-Sir2/SIRT1 Antibody, clone E104, rabbit monoclonal

Cross Reactivity :

Human

Background :

Sir2 is short for Silent mating type Information Regulation-2. Sir2 (whose homolog in mammals is known as SIRT1, SIR2L1 or Sir2α) is the namesake of a family of closely related enzymes, the sirtuins. Members of this family have been found in nearly all organisms studied. Sirtuins are hypothesized to play a key role in an organism's response to stresses (such as heat or starvation) and to be responsible for the lifespan-extending effects of calorie restriction. Sirtuins act by removing acetyl groups from proteins in the presence of NAD+; they are thus classified as "NAD+-dependent deacetylases". In mammals, SIRT1 (the mammalian homolog of Sir2) has been shown to deacetylate and thereby deactivate the p53 protein.

ALT Names :

SIR2-like protein 2;silencing information regulator 2-like ;silent information regulator 2;sir2-related protein type 2;sirtuin (silent mating type information regulation 2;homolog) 2 (S. cerevisiae) ;sirtuin (silent mating type information regulation 2,;S.cerevisiae, homolog) 2;sirtuin 2;sirtuin type 2

Immunogen :

Synthetic peptide corresponding to residues near C-terminus and before Ala-rich domain of human Sir2.

Specificity :

The antibody does not cross-react with other sirtuin family members.

Package Size :

100 µL

Uses :

Immunoprecipitation;Western Blotting;Immunocytochemistry;Immunohistochemistry (Paraffin)

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.