Caspase-3 Antibody, clone E87, rabbit monoclonal | EMD Millipore 04-1090 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Caspase-3 Antibody, clone E87, rabbit monoclonal

catalog :

04-1090

quantity :

100 µL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

E87

The same clone is also sold as:

OriGene: TA300366
Abcam: ab32351

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

citations: 1

Reference
Gomez Pinedo U, Yanez M, Matias Guiu J, Galan L, Guerrero Sola A, Benito Martin M, et al. Cellular changes in motor neuron cell culture produced by cytotoxic cerebrospinal fluid from patients with amyotrophic lateral sclerosis. Neurologia. 2014;29:346-52 pubmed publisher

product information

Catalog Number :

04-1090

Subcategory :

Apoptosis & Cancer

Product Name :

Anti-Caspase-3 Antibody, clone E87, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P42574

Host Name :

Rabbit

Antigen :

Caspase-3

Clone :

E87

Conjugate :

Unpurified

Isotype :

IgG

Product Description :

Anti-Caspase-3 Antibody, clone E87, rabbit monoclonal

Cross Reactivity :

Human;Rat

Background :

Caspase-3 (CPP32, Apopain) is the most extensively studied apoptotic protein. Caspase-3 is synthesized as an inactive proenzyme (32 kDa) that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kDa) and small (12 kDa) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells such as PARP and DFF.

ALT Names :

Cysteine protease CPP32;PARP cleavage protease ;SREBP cleavage activity 1;Yama protein;caspase 3;caspase 3, apoptosis-related cysteine peptidase ;caspase 3, apoptosis-related cysteine protease

Immunogen :

Synthetic peptide corresponding to residues in p17 subunit of human Caspase-3.

Specificity :

The antibody should recognize both pro-form (35 kDa) and p17 cleaved-form of Caspase-3.

Package Size :

100 µL

Uses :

Western Blotting;Immunoprecipitation;Immunohistochemistry (Paraffin);Immunocytochemistry

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.