p53 (NT) Antibody, clone Y5, rabbit monoclonal | EMD Millipore 04-1083 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

p53 (NT) Antibody, clone Y5, rabbit monoclonal

catalog :

04-1083

quantity :

100 µL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

Y5

The same clone is also sold as:

Invitrogen: MA5-14467
Abcam: ab190335, ab32049

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

citations: 3

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 6	Stepanenko A, Andreieva S, Korets K, Mykytenko D, Baklaushev V, Huleyuk N, et al. Temozolomide promotes genomic and phenotypic changes in glioblastoma cells. Cancer Cell Int. 2016;16:36 pubmed publisher
immunoprecipitation; mouse	Pei L, Shang Y, Jin H, Wang S, Wei N, Yan H, et al. DAPK1-p53 interaction converges necrotic and apoptotic pathways of ischemic neuronal death. J Neurosci. 2014;34:6546-56 pubmed publisher
	Kalan S, Matveyenko A, Loayza D. LIM Protein Ajuba Participates in the Repression of the ATR-Mediated DNA Damage Response. Front Genet. 2013;4:95 pubmed publisher

product information

Catalog Number :

04-1083

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-p53 (NT) Antibody, clone Y5, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P04637

Host Name :

Rabbit

Antigen :

p53 (NT)

Clone :

Conjugate :

Unpurified

Isotype :

IgG

Product Description :

Anti-p53 (NT) Antibody, clone Y5, rabbit monoclonal

Cross Reactivity :

Human;Rat

Background :

p53 was discovered in 1979 as a cellular protein associating with the transforming protein of SV40 tumor virus. Since then, many different biochemical functions have been attributed to the 53 kD phosphoprotein. Experimental evidence has suggested that p53 acts as a negative regulator of cell growth in normal cells (Finlay, 1989). Thus, the inactivation or mutation of p53 may be an essential step in the development of malignancy (Lane and Benchmol, 1990). Wild-type p53 levels in normal cells and tissues were found to be very low. Mutant p53 polypeptide, however, is often found to be present at high concentrations in mammalian tumors and tumor cell lines. For example, in an immuno-histochemistry study 40% of human breast cancer showed elevated levels of mutant p53 in the cell nucleus. Mutations of the p53 protein have some characteristic features: a) Most of them are missense point mutations giving rise to an altered protein function. b) Many -but not all- mutant p53 proteins exhibit a common mutant structure, which can be recognized by monoclonal antibodies specific for p53 in the mutant conformation.

ALT Names :

Antigen NY-CO-13;Phosphoprotein p53;Tumor suppressor p53;p53 antigen ;p53 transformation suppressor;p53 tumor suppressor;transformation-related protein 53 ;tumor protein p53

Immunogen :

Synthetic peptide corresponding to residues in the N-terminus of human p53.

Specificity :

The antibody should recognize human wild-type and mutant p53.

Package Size :

100 µL

Uses :

Western Blotting;Immunohistochemistry (Paraffin);Immunocytochemistry;Immunoprecipitation

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.