Anti-Actin Antibody, clone EP184E, rabbit monoclonal | EMD Millipore 04-1040 product information

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Anti-Actin Antibody, clone EP184E, rabbit monoclonal

catalog :

04-1040

quantity :

100 µL

clonality :

monoclonal

host :

rabbit

conjugate :

nonconjugated

clone name :

EP184E

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

EMD Millipore product webpage

citations: 4

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:2000; fig 4	Chen Q, Li R, Zhao X, Liang B, Ma S, Li Z, et al. Prevention of ultraviolet radiation?induced immunosuppression by sunscreen in Candida albicans?induced delayed?type hypersensitivity. Mol Med Rep. 2016;14:202-8 pubmed publisher
	Ruiz de Azua I, Mancini G, Srivastava R, Rey A, Cardinal P, Tedesco L, et al. Adipocyte cannabinoid receptor CB1 regulates energy homeostasis and alternatively activated macrophages. J Clin Invest. 2017;127:4148-4162 pubmed publisher
	Schaeffer V, Hansen K, Morris D, LeBoeuf R, Abrass C. RNA-binding protein IGF2BP2/IMP2 is required for laminin-?2 mRNA translation and is modulated by glucose concentration. Am J Physiol Renal Physiol. 2012;303:F75-82 pubmed publisher
	Adamson R, Frazier A, Evans H, Chambers K, Schenk E, Essand M, et al. In vitro primary cell culture as a physiologically relevant method for preclinical testing of human oncolytic adenovirus. Hum Gene Ther. 2012;23:218-30 pubmed publisher

product information

Catalog Number :

04-1040

Subcategory :

Cell Structure

Product Name :

Anti-Actin Antibody, clone EP184E, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P68032

Host Name :

Rabbit

Antigen :

Actin

Clone :

EP184E

Conjugate :

Unpurified

Isotype :

IgG

Product Description :

Anti-Actin Antibody, clone EP184E, rabbit monoclonal

Cross Reactivity :

Human;Mouse;Rat

Background :

Actin is an abundant cytoskeletal protein found in all cells (True, 1990). The protein's 42 kDa peptide chain assumes two physical forms: globular actin, which may serve as a cytoplasmic storage pool, and fibrous actin, which, in conjunction with myosin, generates muscle contraction (True, 1990). In non-muscle cells, actin appears to be involved in a variety of functions, such as cell motility, exocytosis, and phagocytosis (True, 1990). Distribution of the six known isoforms of actin - four muscle actins (alpha-skeletal, alpha-vascular smooth, alpha-cardiac, and gamma-enteric smooth) and two cytoplasmic actins (alpha and gamma) is tissue specific.

ALT Names :

Alpha-cardiac actin ;actin, alpha, cardiac muscle ;actin, alpha, cardiac muscle 1;cardiac muscle alpha actin 1

Immunogen :

Synthetic peptide corresponding to residues near the N-terminus of human Actin.

Specificity :

This actin antibody recognizes all muscle actin.

Package Size :

100 µL

Uses :

Western Blotting;Immunohistochemistry (Paraffin);Immunocytochemistry;Immunoprecipitation;Flow Cytometry

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

more info or order :

EMD Millipore product webpage