Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

antibody

STAT3 Antibody, clone E121-21, rabbit monoclonal

04-1014

100 µL

monoclonal

domestic rabbit

nonconjugated

E121-21

The same clone is also sold as:

• Abcam: ab32500
• OriGene: TA300380

human, bovine

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Epigenetics & Nuclear Function

Anti-STAT3 Antibody, clone E121-21, rabbit monoclonal

Antibodies

Monoclonal Antibody

P40763

Rabbit

STAT3

E121-21

Unpurified

IgG

Anti-STAT3 Antibody, clone E121-21, rabbit monoclonal

Human

STAT proteins (Signal Transduction and Activators of Transcription) are latent cytoplasmic transcription factors that have the dual function of signal transduction and activation of transcription. STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus. The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue. STATs are a part of the JAK-STAT signaling pathway – a major pathway of the immune system. All cytokines transduce critical signals through this pathway. STAT3 has been shown to be activated by IFN alpha but not IFN beta. The transcription factors associated with STAT3 are cJun and cyclic AMP responsive enhancer binding protein (CREB). Deletion of the STAT3 gene in knock out mice was lethal at the early embryonic stage.

Acute-phase response factor ;DNA-binding protein APRF ;signal transducer and activator of transcription 3;signal transducer and activator of transcription 3 (acute-phase response factor)

Synthetic peptide corresponding to residues surrounding Ser727 of human STAT3.

The antibody only detects STAT3 without phosphorylation on Ser727. It does not detect Ser727-phosphorylated STAT3.

100 µL

Western Blotting;Immunocytochemistry;Immunohistochemistry (Paraffin);Immunoprecipitation

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.