acetyl-Histone H3 (Lys9) Antibody, clone Y28, rabbit monoclonal | EMD Millipore 04-1003 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

acetyl-Histone H3 (Lys9) Antibody, clone Y28, rabbit monoclonal

catalog :

04-1003

quantity :

100 µL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

Y28

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

citations: 5

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; fig s5	Zhang Q, Dan J, Wang H, Guo R, Mao J, Fu H, et al. Tcstv1 and Tcstv3 elongate telomeres of mouse ES cells. Sci Rep. 2016;6:19852 pubmed publisher
western blot; mouse; 1:2000; fig 3	Jamaladdin S, Kelly R, O Regan L, Dovey O, Hodson G, Millard C, et al. Histone deacetylase (HDAC) 1 and 2 are essential for accurate cell division and the pluripotency of embryonic stem cells. Proc Natl Acad Sci U S A. 2014;111:9840-5 pubmed publisher
immunocytochemistry; mouse; 1:300	Ma P, Pan H, Montgomery R, Olson E, Schultz R. Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development. Proc Natl Acad Sci U S A. 2012;109:E481-9 pubmed publisher
	Dangi Garimella S, Sahai V, Ebine K, Kumar K, Munshi H. Three-dimensional collagen I promotes gemcitabine resistance in vitro in pancreatic cancer cells through HMGA2-dependent histone acetyltransferase expression. PLoS ONE. 2013;8:e64566 pubmed publisher
	Kenworthy C, Sengupta A, Luz S, Ver Hoeve E, Meda K, Bhatnagar S, et al. Social defeat induces changes in histone acetylation and expression of histone modifying enzymes in the ventral hippocampus, prefrontal cortex, and dorsal raphe nucleus. Neuroscience. 2014;264:88-98 pubmed publisher

product information

Catalog Number :

04-1003

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-acetyl-Histone H3 (Lys9) Antibody, clone Y28, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P84243

Host Name :

Rabbit

Antigen :

acetyl-Histone H3 (Lys9)

Clone :

Y28

Conjugate :

Unpurified

Isotype :

IgG

Product Description :

Anti-acetyl-Histone H3 (Lys9) Antibody, clone Y28, rabbit monoclonal

Cross Reactivity :

Human;Mouse

Background :

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

ALT Names :

H3K9Ac;Histone H3 (acetyl K9);H3 histone, family 3A

Immunogen :

Acetylated-synthetic peptide corresponding to residues surrounding Lys9 of Histone H3.

Specificity :

The antibody only detects Histone H3 acetylated on Lys9.

Package Size :

100 µL

Uses :

Western Blotting;Immunocytochemistry;Immunohistochemistry (Paraffin);Immunoprecipitation

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.