β-Catenin (NT) Antibody, clone E247, rabbit monoclonal | EMD Millipore 04-1002 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

β-Catenin (NT) Antibody, clone E247, rabbit monoclonal

catalog :

04-1002

quantity :

100 μL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

E247

The same clone is also sold as:

GeneTex: GTX61089
Abcam: ab194120, ab194118, ab32572
Invitrogen: MA5-14461
OriGene: AM10048SU-N

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

citations: 1

Reference
Sasaki M, Yoneda N, Kitamura S, Sato Y, Nakanuma Y. Characterization of hepatocellular adenoma based on the phenotypic classification: The Kanazawa experience. Hepatol Res. 2011;41:982-8 pubmed publisher

product information

Catalog Number :

04-1002

Subcategory :

Cell Signaling

Product Name :

Anti-β-Catenin (NT) Antibody, clone E247, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P35222

Host Name :

Rabbit

Antigen :

β-Catenin (NT)

Clone :

E247

Conjugate :

Unpurified

Isotype :

IgG

Product Description :

Anti-β-Catenin (NT) Antibody, clone E247, rabbit monoclonal

Cross Reactivity :

Human;Mouse

Background :

Β-Catenin links Cadherin cell adhesion proteins to the cytoskeleton and has recently been implicated in the Wnt/Wingless signaling pathway and in the progression of neoplasia. β-Catenin associates with the tumor suppressor Adenomatous Polypopsis Coli (APC) and functions as a co-activator of the TCF transcription factor family. It is regulated by Glycogen Synthase Kinase (GSK) 3b, which phosphorylates and targets β-Catenin for ubiquitin-mediated proteolysis. Stabilization of β-Catenin, either by Wnt-mediated inhibition of GSK3b or by mutation (as found in numerous colorectal tumors and melanoma cell lines), results in activation of TCF responsive genes which are thought to play key roles in development and cancer progression. δ-Catenin (NPRAP/Neurojungin), a member of the p120 Catenin subfamily, is a structural protein involved in neuronal development. It interacts with Actin, Cortactin and Src Kinases to promote neurite elongation or branching, depending upon its phosphorylation state.

ALT Names :

catenin (cadherin-associated protein), beta 1 (88kD) ;catenin (cadherin-associated protein), beta 1, 88kDa;catenin beta-1

Immunogen :

Synthetic peptide corresponding to residues near the N-terminus of human β-catenin.

Specificity :

This antibody recognizes β-catenin at and around the N-terminus.

Package Size :

100 μL

Uses :

Immunohistochemistry;Western Blotting;Immunocytochemistry;Immunoprecipitation

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.