trimethyl-Histone H4 (Lys20), Antibody, rabbit monoclonal | EMD Millipore 04-079 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

trimethyl-Histone H4 (Lys20), Antibody, rabbit monoclonal

catalog :

04-079

quantity :

100 µL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunocytochemistry, chromatin immunoprecipitation, immunohistochemistry - paraffin section

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; fig 1 chromatin immunoprecipitation; human; fig s1 immunocytochemistry; human; fig 1 western blot; human; fig 1	Nelson D, Jaber Hijazi F, Cole J, Robertson N, Pawlikowski J, Norris K, et al. Mapping H4K20me3 onto the chromatin landscape of senescent cells indicates a function in control of cell senescence and tumor suppression through preservation of genetic and epigenetic stability. Genome Biol. 2016;17:158 pubmed publisher

product information

Catalog Number :

04-079

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-trimethyl-Histone H4 (Lys20), Antibody, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P62805

Host Name :

Rabbit

Antigen :

trimethyl-Histone H4 (Lys20)

Conjugate :

Culture Supernatant

Isotype :

IgG

Product Description :

Anti-trimethyl-Histone H4 (Lys20), Antibody, rabbit monoclonal

Cross Reactivity :

Human;Vertebrates

Background :

Histones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli, including phosphorylation on serine and threonine residues and methylation on lysine residues. PTMs produce configural changes in histone proteins that may induce nucleosome remodeling and expose or hide DNA sequences from transcriptional complexes. Histone H4 lysine 20 (H4K20) may undergo mono-, di-, or trimethylation, which is catalyzed by the methyltransferase PR-Set7 (Set8 or KMT5a). Methylated H4K20 plays a role in regulating DNA damage responses, mitosis, DNA replication, and gene expression. Trimethylation of H4K20 contributes to gene silencing, and is a mark of the repressive heterochromatin state.

ALT Names :

H4K20me3;Histone H4 (tri methyl K20)

Immunogen :

Peptide corresponding to human Histone H4 containing the sequence [HRmethKVL] on which lysine 20 is trimethylated.

Specificity :

Recognizes Histone H4 when trimethylated on Lys20.

Package Size :

100 µL

Uses :

Western Blotting;Dot Blot;Chromatin Immunoprecipitation (ChIP)

Storage :

2 years at -20°C from date of shipment