PTEN Antibody, clone 6H2.1 | EMD Millipore 04-035 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

PTEN Antibody, clone 6H2.1

catalog :

04-035

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6H2.1

The same clone is also sold as:

OriGene: AM10137SU-N
Dako: M3627

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

citations: 14

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 6	HÃ¸ye A, Couchman J, Wewer U, Yoneda A. The Phosphorylation and Distribution of Cortactin Downstream of Integrin Î±9Î²1 Affects Cancer Cell Behaviour. Sci Rep. 2016;6:28529 pubmed publisher
western blot; human; fig 3	Tzani I, Ivanov I, Andreev D, Dmitriev R, Dean K, Baranov P, et al. Systematic analysis of the PTEN 5' leader identifies a major AUU initiated proteoform. Open Biol. 2016;6: pubmed publisher
western blot; human; loading ...; fig e7c	Saeed M, Andreo U, Chung H, Espiritu C, Branch A, Silva J, et al. SEC14L2 enables pan-genotype HCV replication in cell culture. Nature. 2015;524:471-5 pubmed publisher
western blot; mouse; 1:1000; fig 5	Zhou W, Shao H, Zhang D, Dong J, Cheng W, Wang L, et al. PTEN signaling is required for the maintenance of spermatogonial stem cells in mouse, by regulating the expressions of PLZF and UTF1. Cell Biosci. 2015;5:42 pubmed publisher
western blot; mouse	Perez García V, Redondo Muñoz J, Kumar A, Carrera A. Cell activation-induced phosphoinositide 3-kinase alpha/beta dimerization regulates PTEN activity. Mol Cell Biol. 2014;34:3359-73 pubmed publisher
immunohistochemistry - frozen section; human; 1:100 western blot; human; 1:100	Screen M, Jonson P, Raheem O, Palmio J, Laaksonen R, Lehtimaki T, et al. Abnormal splicing of NEDD4 in myotonic dystrophy type 2: possible link to statin adverse reactions. Am J Pathol. 2014;184:2322-32 pubmed publisher
	Vuono E, Mukherjee A, Vierra D, Adroved M, Hodson C, Deans A, et al. The PTEN phosphatase functions cooperatively with the Fanconi anemia proteins in DNA crosslink repair. Sci Rep. 2016;6:36439 pubmed publisher
	Li J, Tanhehco E, Russell B. Actin dynamics is rapidly regulated by the PTEN and PIP2 signaling pathways leading to myocyte hypertrophy. Am J Physiol Heart Circ Physiol. 2014;307:H1618-25 pubmed publisher
	Trietsch M, Spaans V, Ter Haar N, Osse E, Peters A, Gaarenstroom K, et al. CDKN2A(p16) and HRAS are frequently mutated in vulvar squamous cell carcinoma. Gynecol Oncol. 2014;135:149-55 pubmed publisher
	Park R, Chen J, Kim J, Jeong S, Ohn T. Splicing factor SRSF3 represses the translation of programmed cell death 4 mRNA by associating with the 5'-UTR region. Cell Death Differ. 2014;21:481-90 pubmed publisher
	Jones A, Teschendorff A, Li Q, Hayward J, Kannan A, Mould T, et al. Role of DNA methylation and epigenetic silencing of HAND2 in endometrial cancer development. PLoS Med. 2013;10:e1001551 pubmed publisher
	ANASTASIO N, Gilbertson S, Bubar M, Agarkov A, Stutz S, Jeng Y, et al. Peptide inhibitors disrupt the serotonin 5-HT2C receptor interaction with phosphatase and tensin homolog to allosterically modulate cellular signaling and behavior. J Neurosci. 2013;33:1615-30 pubmed publisher
	Li F, Shen L, Li Z, Luo H, Qiu M, Zhang H, et al. Impact of KRAS mutation and PTEN expression on cetuximab-treated colorectal cancer. World J Gastroenterol. 2010;16:5881-8 pubmed
	Lobo G, Waite K, Planchon S, Romigh T, Nassif N, Eng C. Germline and somatic cancer-associated mutations in the ATP-binding motifs of PTEN influence its subcellular localization and tumor suppressive function. Hum Mol Genet. 2009;18:2851-62 pubmed publisher

product information

Catalog Number :

04-035

Subcategory :

Cell Signaling

Product Name :

Anti-PTEN Antibody, clone 6H2.1

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P60484;O08586 (mouse)

Host Name :

Mouse

Antigen :

PTEN

Clone :

6H2.1

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-PTEN Antibody, clone 6H2.1

Cross Reactivity :

Human;Mouse;Rat

Background :

The protein and lipid phosphatase PTEN (phosphatase and tensin homolog deleted on chromosome ten) has been suggested to play a role in tumorgenesis. PTEN is a tumor suppressor whose loss permits constitutive signaling through the PI3K pathway and is sufficient to cause cancer. In normal cells, in conjunction with FOXO1A and PTEN function together to restrain cell growth. PTEN metabolizes the product of PI3K phosphorylation of PI(4,5)P2 following receptor activation, creating PI(3,4,5)P3. In cells lacking PTEN, there are elevated levels of PI(3,4,5)P3, which acts as a potent second messenger promoting oncogenesis. Restoration of PTEN function is considered a novel approach to cancer therapy. Overexpression of PTEN inhibits cell migration, and expression of antisense PTEN mRNA enhances migration.

Immunogen :

C-terminal 100 amino acids of PTEN

Specificity :

recognizes PTEN

Package Size :

100 μg

Uses :

Western Blotting;Immunoprecipitation;Immunohistochemistry (Paraffin)

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.