RIPAb+™ Ago3 Antibody | EMD Millipore 03-250 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

RIPAb+™ Ago3 Antibody

catalog :

03-250

quantity :

10 assays

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human

application :

immunocytochemistry, immunoprecipitation

citations: 1

Reference
Hussain S, Sajini A, Blanco S, Dietmann S, Lombard P, Sugimoto Y, et al. NSun2-mediated cytosine-5 methylation of vault noncoding RNA determines its processing into regulatory small RNAs. Cell Rep. 2013;4:255-61 pubmed publisher

product information

Catalog Number :

03-250

Subcategory :

Epigenetics & Nuclear Function

Product Name :

RIPAb+™ Ago3 Antibody

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q9H9G7

Host Name :

Mouse

Antigen :

Ago3

Conjugate :

Purified

Isotype :

IgG2aκ

Product Description :

RIPAb+™ Ago3 Antibody

Cross Reactivity :

Human

Background :

Eukaryotic translation initiation factor 2C 3, Ago3, belongs to the argonaute family and contains 1 PAZ and 1 Piwi domain. Ago3 is indicated to play a role in the RNA interference (RNAi) pathway, which is important for heterochromatin formation and accurate chromosome segregation. Unlike Ago1 and Ago2, Ago3 and AG04 do not possess the ability to dissociate miRNA duplexes in a minimal in vitro system. Re-introduction of any Ago variant into embyronic (ES) cells deficient for expression of all four Ago variants will rescue miRNA silencing defects and reduce apoptosis.

ALT Names :

eIF-2C 3;eIF2C 3;FLJ12765;MGC86946

Immunogen :

Recombinant protein corresponding to human Ago3

Specificity :

This antibody has been shown to not cross react with Ago1, Ago2, or Ago4 (Azuma-Mukai A et al. (2008) Proc. Natl. Acad. Sci. USA 105: 7964-7969).

Package Size :

10 assays

Uses :

Immunocytochemistry;Immunoprecipitation;RNA Binding Protein Immunoprecipitation (RIP)

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.