RIPAb+™ Ago1 Antibody | EMD Millipore 03-249 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

RIPAb+™ Ago1 Antibody

catalog :

03-249

quantity :

10 assays

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human

application :

western blot

product information

Catalog Number :

03-249

Subcategory :

Epigenetics & Nuclear Function

Product Name :

RIPAb+™ Ago1 Antibody

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q9UL18

Host Name :

Mouse

Antigen :

Ago1

Conjugate :

Purified

Isotype :

IgG2aκ

Product Description :

RIPAb+™ Ago1 Antibody

Cross Reactivity :

Human

Background :

Ago1 (Eukaryotic translation initiation factor 2C 1) belongs to the argonaute family and contains 1 PAZ and 1 Piwi domain. It is required for G1 arrest and mating in response to nitrogen starvation. It is also known as a cell cycle control protein through its regulation of cytokinesis and its related cell cycle checkpoints, which occur downstream of dcr1. It is required, indirectly, for regulated hyperphosphorylation of cdc2. Ago 1 also has a role in the RNA interference (RNAi) pathway, which is important for heterochromatin formation and accurate chromosome segregation. It has been shown to be non-essential for siRNA-directed target RNA cleavage, but is needed for the production of mature miRNA that affects miRNA-directed RNA cleavage.

ALT Names :

hAgo1;Argonaute1;eIF-2C 1;eIF2C 1;Q99;GERP95;DKFZp686M13167

Immunogen :

GST-tagged recombinant protein corresponding to human Ago1

Specificity :

This antibody has been shown by an independent laboratory to not cross react with Ago2, Ago3, or Ago4 (Azuma-Mukai A et al. (2008) Proc. Natl. Acad. Sci. USA 105: 7964-7969).

Package Size :

10 assays

Uses :

Western Blotting;RNA Binding Protein Immunoprecipitation (RIP)

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.