RIPAb+™ pan Ago - RIP Validated Antibody and Primer Set | EMD Millipore 03-248 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

RIPAb+™ pan Ago - RIP Validated Antibody and Primer Set

catalog :

03-248

quantity :

10 assays

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

product information

Catalog Number :

03-248

Subcategory :

Epigenetics & Nuclear Function

Product Name :

RIPAb+™ pan Ago - RIP Validated Antibody and Primer Set

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q9UKV8

Host Name :

Mouse

Antigen :

pan Ago

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

RIPAb+™ pan Ago - RIP Validated Antibody and Primer Set

Cross Reactivity :

Human

Background :

Argonaute (Ago) proteins are a family of proteins of ~95 kDa that bind directly to mature miRNAs. In mammals, there are four Ago proteins (Ago1-4). This family of proteins is a central component of most mammalian miRNPs that mediates important functions in small RNA-directed regulatory pathways. The Ago family consists of two subclases, Ago and Piwi containing two characteristic domains known as PAZ and PIWI. These two domains interact with different parts of the mature miRNA. The PAZ domain interacts with the 3’-end of the miRNA, and the PIWI domain interacts with the 5’ phosphate and 5’-proximal region of the miRNA to guide target mRNA recognition.

ALT Names :

eukaryotic translation initiation factor 2C, 2;protein argonaute-2;argonaute 2;Protein slicer;argonaute2;PAZ Piwi domain protein;Eukaryotic translation initiation factor 2C;Argonaute2;Argonaute4;Ago4;Protein argonaute-4

Immunogen :

Histidine - tagged recombinant protein corresponding to human Ago.

Specificity :

This antibody recognizes Ago1, Ago2, Ago3, and Ago4. Note that this antibody has been reported to cross-react with radixin (~70 kDa). (Nelson, P., et al. (2007). RNA. 13:1787–1792.)

Package Size :

10 assays

Uses :

Western Blotting;Immunoprecipitation;Immunocytochemistry;Immunohistochemistry;RNA Binding Protein Immunoprecipitation (RIP)

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.